Brown Roger F, Day Delbert E, Day Thomas E, Jung Steve, Rahaman Mohamed N, Fu Qiang
Departments of Biological Sciences, University of Missouri - Rolla, Rolla, MO 65409-1120, USA.
Acta Biomater. 2008 Mar;4(2):387-96. doi: 10.1016/j.actbio.2007.07.006. Epub 2007 Jul 28.
This in vitro study was conducted to evaluate the ability of two types of constructs of bioactive, silica-based 13-93 glass fibers to support the growth and differentiation of MC3T3-E1 osteoblastic cells. The two types of constructs tested included single-layer 13-93 glass fiber rafts and three-dimensional porous scaffolds formed from sintered 13-93 fibers. Scanning electron micrographs showed a closely adhering, well-spread morphology of MC3T3-E1 cells seeded on both types of constructs. The scanning electron microscopy images also showed a continuous increase in cell densities during a 6 day incubation on 13-93 glass fiber rafts and scaffolds. Quantitative fluorescence measurements of DNA also revealed a linear increase in cell density during a 6 day incubation on both types of 13-93 constructs. Examination of scaffolds incubated in MTT containing medium showed the presence of metabolically active viable cells within the interior of the scaffold. The addition of ascorbic acid to MC3T3-E1 cells cultured on the 13-93 glass fibers triggered a threefold increase in alkaline phosphatase, a key indicator of osteoblast differentiation. The sintered scaffolds were found to have open, interconnected pores favorable for tissue ingrowth with a compressive strength similar to cancellous bone. Collectively, the results indicate that 13-93 glass fiber scaffolds are a favorable substrate for the growth and differentiation of osteoblasts and a promising material for bone tissue engineering and repair of bone defects.
本体外研究旨在评估两种具有生物活性的二氧化硅基13-93玻璃纤维构建体支持MC3T3-E1成骨细胞生长和分化的能力。所测试的两种构建体包括单层13-93玻璃纤维筏和由烧结13-93纤维形成的三维多孔支架。扫描电子显微镜图像显示，接种在这两种构建体上的MC3T3-E1细胞形态紧密附着且铺展良好。扫描电子显微镜图像还显示，在13-93玻璃纤维筏和支架上培养6天期间，细胞密度持续增加。DNA的定量荧光测量也显示，在两种13-93构建体上培养6天期间，细胞密度呈线性增加。对在含MTT培养基中培养的支架进行检查，结果显示支架内部存在代谢活跃的活细胞。向在13-93玻璃纤维上培养的MC3T3-E1细胞中添加抗坏血酸，引发了碱性磷酸酶增加三倍，碱性磷酸酶是成骨细胞分化的关键指标。发现烧结支架具有开放的、相互连接的孔隙，有利于组织向内生长，其抗压强度与松质骨相似。总体而言，结果表明13-93玻璃纤维支架是成骨细胞生长和分化的良好基质，是骨组织工程和骨缺损修复的有前景的材料。
