Chen Q Z, Efthymiou A, Salih V, Boccaccini A R
Department of Materials, Imperial College London, Prince Consort Road, London SW7 2BP, United Kingdom.
J Biomed Mater Res A. 2008 Mar 15;84(4):1049-60. doi: 10.1002/jbm.a.31512.
Cell support function as well as cell proliferation on highly porous Bioglass(R)-derived glass-ceramic scaffolds (designed for bone tissue engineering) have been assessed in vitro using osteoblast-like cells (MG 63) cultured for up to 6 days. The biodegradation and mechanical stability of the scaffolds in the cell-culture medium have also been investigated. It was found that the scaffolds had excellent cell supporting ability, with cells effectively infiltrating into and surviving at the center of the scaffolds. A quantitative study using the AlamarBlue assay revealed that the proliferation of cells on the glass-ceramic materials was comparable to that on the noncrystallized Bioglass. While the crystalline phase in the glass-ceramic scaffolds transformed into a biodegradable amorphous calcium phosphate phase during cell culture, the mechanical strength of the scaffolds was maintained when compared with that of scaffolds incubated in simulated body fluid or immersed in cell-free culture medium. It is believed that the attached cells and collagen secreted by cells could fill the micropores and microcracks on the surface of the foam struts, thus contributing to the mechanical stability of the degrading scaffolds. In summary, the developed glass-ceramic scaffolds possess the most essential features of a scaffold for bone tissue engineering: they are capable to support and foster relevant cells, able to provide temporary mechanical function, and biodegradable.
使用成骨样细胞（MG 63）在体外培养长达6天，对高度多孔的生物活性玻璃衍生的微晶玻璃支架（专为骨组织工程设计）的细胞支持功能以及细胞增殖进行了评估。还研究了支架在细胞培养基中的生物降解和机械稳定性。结果发现，这些支架具有出色的细胞支持能力，细胞能够有效渗透到支架中心并在其中存活。使用AlamarBlue检测法进行的定量研究表明，玻璃陶瓷材料上细胞的增殖与非晶态生物活性玻璃上的细胞增殖相当。虽然在细胞培养过程中，玻璃陶瓷支架中的晶相转变为可生物降解的无定形磷酸钙相，但与在模拟体液中孵育或浸入无细胞培养基中的支架相比，支架的机械强度得以保持。据信，附着的细胞和细胞分泌的胶原蛋白可以填充泡沫支柱表面的微孔和微裂纹，从而有助于降解支架的机械稳定性。总之，所开发的玻璃陶瓷支架具备骨组织工程支架最基本的特征：它们能够支持和促进相关细胞生长，能够提供临时机械功能，并且可生物降解。
