Gonzalo-Juan Isabel, Detsch Rainer, Mathur Sanjay, Ionescu Emanuel, Boccaccini Aldo R, Riedel Ralf
FB Material-und Geowissenschaften, Technische Universität Darmstadt, Jovanka-Bontschits-Strasse 2, Darmstadt D-64287, Germany.
Department of Materials Science and Engineering, Institute of Biomaterials, University of Erlangen-Nuremberg, Cauerstrasse 6, Erlangen D-91058, Germany.
Materials (Basel). 2016 Nov 24;9(12):959. doi: 10.3390/ma9120959.
Novel bioactive glasses based on a Ca- and Mg-modified silicon oxycarbide (SiCaMgOC) were prepared from a polymeric single-source precursor, and their in vitro activity towards hydroxyapatite mineralization was investigated upon incubating the samples in simulated body fluid (SBF) at 37 °C. The as-prepared materials exhibit an outstanding resistance against devitrification processes and maintain their amorphous nature even after exposure to 1300 °C. The X-ray diffraction (XRD) analysis of the SiCaMgOC samples after the SBF test showed characteristic reflections of apatite after only three days, indicating a promising bioactivity. The release kinetics of the Ca and Mg and the adsorption of H⁺ after immersion of SiCaMgOC in simulated body fluid for different soaking times were analyzed via optical emission spectroscopy. The results show that the mechanism of formation of apatite on the surface of the SiCaMgOC powders is similar to that observed for standard (silicate) bioactive glasses. A preliminary cytotoxicity investigation of the SiOC-based bioactive glasses was performed in the presence of mouse embryonic fibroblasts (MEF) as well as human embryonic kidney cells (HEK-293). Due to their excellent high-temperature crystallization resistance in addition to bioactivity, the Ca- and Mg-modified SiOC glasses presented here might have high potential in applications related to bone repair and regeneration.
基于钙和镁改性的碳氧化硅（SiCaMgOC）制备了新型生物活性玻璃，其由聚合物单源前驱体制备而成，并通过在37°C的模拟体液（SBF）中孵育样品，研究了它们对羟基磷灰石矿化的体外活性。所制备的材料对失透过程表现出优异的抗性，即使在暴露于1300°C后仍保持其非晶态性质。SBF测试后SiCaMgOC样品的X射线衍射（XRD）分析表明，仅三天后就出现了磷灰石的特征反射，表明具有良好的生物活性。通过光发射光谱分析了SiCaMgOC在模拟体液中浸泡不同时间后钙和镁的释放动力学以及H⁺的吸附情况。结果表明，SiCaMgOC粉末表面磷灰石的形成机制与标准（硅酸盐）生物活性玻璃所观察到的机制相似。在小鼠胚胎成纤维细胞（MEF）以及人胚肾细胞（HEK-293）存在的情况下，对基于SiOC的生物活性玻璃进行了初步细胞毒性研究。由于此处呈现的钙和镁改性SiOC玻璃除了具有生物活性外还具有优异的高温抗结晶性，它们在与骨修复和再生相关的应用中可能具有很高的潜力。
