变形链球菌葡聚糖葡萄糖苷酶的结晶及初步X射线分析
Crystallization and preliminary X-ray analysis of Streptococcus mutans dextran glucosidase.
作者信息
Saburi Wataru, Hondoh Hironori, Unno Hideaki, Okuyama Masayuki, Mori Haruhide, Nakada Toshitaka, Matsuura Yoshiki, Kimura Atsuo
机构信息
Research Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido 060-8589, Japan.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Sep 1;63(Pt 9):774-6. doi: 10.1107/S174430910703936X. Epub 2007 Aug 25.
Abstract
Dextran glucosidase from Streptococcus mutans is an exo-hydrolase that acts on the nonreducing terminal alpha-1,6-glucosidic linkage of oligosaccharides and dextran with a high degree of transglucosylation. Based on amino-acid sequence similarity, this enzyme is classified into glycoside hydrolase family 13. Recombinant dextran glucosidase was purified and crystallized by the hanging-drop vapour-diffusion technique using polyethylene glycol 6000 as a precipitant. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 72.72, b = 86.47, c = 104.30 A. A native data set was collected to 2.2 A resolution from a single crystal.
摘要
变形链球菌葡聚糖葡糖苷酶是一种外切水解酶，作用于寡糖和葡聚糖的非还原末端α-1,6-糖苷键，具有高度的转糖基化作用。基于氨基酸序列相似性，该酶被归类于糖苷水解酶家族13。使用聚乙二醇6000作为沉淀剂，通过悬滴气相扩散技术对重组葡聚糖葡糖苷酶进行了纯化和结晶。晶体属于正交晶系空间群P2(1)2(1)2(1)，晶胞参数a = 72.72，b = 86.47，c = 104.30 Å。从单晶收集到分辨率为2.2 Å的天然数据集。
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