利用原光节杆菌内切β-N-乙酰氨基葡萄糖苷酶的转糖基化活性酶促合成新型寡糖。
Enzymatic synthesis of novel oligosaccharides by use of transglycosylation activity of endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae.
作者信息
Takegawa K, Yamaguchi S, Kondo A, Kato I, Iwahara S
机构信息
Department of Bioresource Science, Faculty of Agriculture, Kagawa University, Japan.
出版信息
Biochem Int. 1991 Dec;25(5):829-35.
The transglycosylation activity of endo-beta-N-acetylglucosaminidase from Arthrobacter protophormiae was used for the enzymatic synthesis of novel oligosaccharides. When (Man)6(GlcNAc)2Asn was used as a substrate for the transglycosylation, (Man)6GlcNAc-Glc, (Man)6GlcNAc-Man, (Man)6GlcNAc-chitobiose, and (Man)6GlcNAc-gentiobiose were synthesized. Their structures were identified by HPLC, ion spray mass spectrometry, and digestion with glycosidases. Endo-beta-N-acetylglucosaminidases hydrolyzed the pyridylamino derivatives of these oligosaccharides.
原噬菌体节杆菌的内切β-N-乙酰氨基葡萄糖苷酶的转糖基化活性被用于新型寡糖的酶促合成。当以(Man)6(GlcNAc)2Asn作为转糖基化的底物时，合成了(Man)6GlcNAc-Glc、(Man)6GlcNAc-Man、(Man)6GlcNAc-壳二糖和(Man)6GlcNAc-龙胆二糖。通过高效液相色谱法、离子喷雾质谱法以及糖苷酶消化对它们的结构进行了鉴定。内切β-N-乙酰氨基葡萄糖苷酶水解了这些寡糖的吡啶氨基衍生物。
Zotero 插件