Hillyard S D
J Exp Zool. 1976 Feb;195(2):199-206. doi: 10.1002/jez.1401950204.
The rate of active sodium transport as measured by short-circuit current across the isolated skin of the toad, Scaphiopus couchi, was elevated following vasopressin (0.2 units/ml) or arginine vasotocin (0.1 units/ml) treatment of skins from active animals at all times of the year tested. Skins from dormant animals showed no such elevation at any time of the year. The rate of active sodium transport was elevated following treatment with dibutyryl cyclic AMP (2.5mM) plus theophylline (10 mM) in all skins tested. The hydraulic conductivity of isolated skins from both active and dormant animals showed no significant change following treatment with vasopressin (0.2 units/ml) or arginine vasotocin (0.1 units/ml except on the first day following emergence from dormancy in the field. A correlation was, therefore, observed between the occurrence of a hydroosmotic response to antidiuretic hormones and the seasonal exposure of S. couchi to standing water. A small but significant elevation of hydraulic conductivity was observed across the skins of dormant toads following treatment with dibutyryl cyclic AMP (2.5 mM) plus theophylline (10 mM) whereas a substantial elevation was observed with the skins of active animals.
通过测量蟾蜍（库奇锄足蟾，Scaphiopus couchi）离体皮肤的短路电流来测定主动钠转运速率，在全年测试的任何时间，用抗利尿激素（0.2单位/毫升）或精氨酸血管加压素（0.1单位/毫升）处理处于活跃期动物的皮肤后，主动钠转运速率都会升高。处于休眠期动物的皮肤在一年中的任何时候都未显示出这种升高。在用二丁酰环磷腺苷（2.5毫摩尔）加茶碱（10毫摩尔）处理的所有测试皮肤中，主动钠转运速率均升高。在用抗利尿激素（0.2单位/毫升）或精氨酸血管加压素（0.1单位/毫升）处理后，活跃期和休眠期动物的离体皮肤的水导率均未显示出显著变化，但在野外从休眠中苏醒后的第一天除外。因此，观察到抗利尿激素的水渗透反应的发生与库奇锄足蟾季节性接触静水之间存在相关性。在用二丁酰环磷腺苷（2.5毫摩尔）加茶碱（10毫摩尔）处理后，休眠蟾蜍的皮肤水导率有小幅但显著的升高，而活跃期动物的皮肤水导率则有大幅升高。
