[环孢素A抑制蟾蜍膀胱对抗利尿激素以及蟾蜍皮肤对血管紧张素II和抗利尿激素的渗透水通透性反应]

[Cyclosporin A inhibits the response of osmotic water permeability to antidiuretic hormone in toad's bladder and to angiotensin II and antidiuretic hormone in toad's skin].

作者信息

Santos J C, Peral de Bruno M, Jerez S, Coviello A

机构信息

Fundación INELCO, Facultad de Ciencias Naturales e Instituto Miguel Lillo, Tucumán, Argentina.

出版信息

Nefrologia. 2001 Jan-Feb;21(1):59-64.

PMID:11344963

Abstract

The purpose of the present study was to assess the effects of cyclosporine A (CyA) in the osmotic water flow response of isolated toad bladder to arginine-vasotocin (AVT) and to angiotensin II (Ang II) and AVT in isolated toad skin. CyA added to the dermal side of isolated toad skin or to the serosal side of toad bladder in concentrations of 0.42. 10(-6) M to 0.42. 10(-7) M had no effect on basal osmotic water permeability (Posm) but inhibited the hormonal response to AVT in both membranes (AVT 10(-10) M in toad bladder and 10(-8) to 10(-9) M in toad skin). CyA also inhibited the Posm response to Ang II (10(-7) M) in toad skin in concentrations of 0.42. 10(-6) M and 0.42. 10(-7) M. In toad bladder it could be demonstrated that the inhibitory effect was reversible. CyA in concentrations of 0.42. 10(-6) M inhibited the Posm response of toad skin to theophylline (3.2. 10(-3) M) and to dibutyryl cyclic AMP (6.3. 10(-3) M) suggesting an effect distal to the generation of cyclic AMP. These responses would support the possibility of a diuretic effect in the mammalian nephron.

摘要

本研究的目的是评估环孢素A（CyA）对离体蟾蜍膀胱对精氨酸血管加压素（AVT）以及对离体蟾蜍皮肤中血管紧张素II（Ang II）和AVT的渗透水流反应的影响。以0.42×10⁻⁶ M至0.42×10⁻⁷ M的浓度添加到离体蟾蜍皮肤真皮侧或蟾蜍膀胱浆膜侧的CyA，对基础渗透水通透性（Posm）没有影响，但抑制了两种膜对AVT的激素反应（蟾蜍膀胱中为10⁻¹⁰ M的AVT，蟾蜍皮肤中为10⁻⁸至10⁻⁹ M的AVT）。CyA还以0.42×10⁻⁶ M和0.42×10⁻⁷ M的浓度抑制蟾蜍皮肤对Ang II（10⁻⁷ M）的Posm反应。在蟾蜍膀胱中可以证明这种抑制作用是可逆的。浓度为0.42×10⁻⁶ M的CyA抑制蟾蜍皮肤对茶碱（3.2×10⁻³ M）和二丁酰环磷腺苷（6.3×10⁻³ M）的Posm反应，这表明在环磷腺苷生成的远端存在作用。这些反应支持了在哺乳动物肾单位中产生利尿作用的可能性。