Singh A, Reinhart W H
Department of Internal Medicine, University of Bern, Inselspital, Switzerland.
Eur J Clin Invest. 1991 Dec;21(6):597-600. doi: 10.1111/j.1365-2362.1991.tb01415.x.
Erythrocytes with abnormal shapes and deformability reduce cell aggregation and hence sedimentation. It is not known what influence small fractions of these abnormal cells have on the sedimentation behaviour of normal cells. We have used three different methods to alter erythrocyte properties (glutaraldehyde, heat treatment, and metabolic depletion). The admixture of such cells in increasing fractions to normal cells affected the sedimentation behaviour in three different ways. Heat-treated erythrocytes decreased the sedimentation proportionally. Small fractions of glutaraldehyde-treated cells increased the sedimentation, which was explained by a pro-aggregatory change of surface properties by glutaraldehyde. With 50% or more glutaraldehyde-treated cells sedimentation was inhibited, which was due to excessive formation of aggregates occupying the entire tube. The presence of small fractions of echinocytes produced by metabolic depletion decreased cell sedimentation disproportionally, which was explained by an inability of spiculated cells to form aggregates with normal erythrocytes. These results indicate that erythrocyte sedimentation is affected by fraction of abnormal cells in a complex way. Many diseases are characterized by fractions of abnormal erythrocytes. Our results may, therefore, contribute to a better understanding of these conditions.
形状和可变形性异常的红细胞会减少细胞聚集，从而降低沉降率。目前尚不清楚这些异常细胞的小部分对正常细胞沉降行为有何影响。我们使用了三种不同的方法来改变红细胞特性（戊二醛、热处理和代谢消耗）。将这些细胞以递增比例与正常细胞混合，以三种不同方式影响沉降行为。热处理的红细胞按比例降低沉降率。小部分经戊二醛处理的细胞会增加沉降率，这可通过戊二醛使表面性质发生促聚集变化来解释。当经戊二醛处理的细胞达到50%或更多时，沉降受到抑制，这是由于聚集体过度形成占据了整个试管。代谢消耗产生的小部分棘形红细胞的存在会不成比例地降低细胞沉降率，这可通过有刺细胞无法与正常红细胞形成聚集体来解释。这些结果表明，红细胞沉降受异常细胞比例的影响方式很复杂。许多疾病的特征是存在异常红细胞比例。因此，我们的结果可能有助于更好地理解这些情况。
