Amano K, Watanabe M, Takimoto H, Kumazawa Y
Central Research Laboratory, Akita University School of Medicine, Japan.
Dev Biol Stand. 1991;73:193-9.
Lipopolysaccharides (LPS) isolated from Bordetella pertussis (Bp), B. parapertussis (Bpp), and B. bronchiseptica (Bbs) were analysed for their chemical composition, molecular heterogeneity, and immunological and biological properties. All LPS contained heptose, KDO, GlcN, uronic acid, phosphate, and fatty acids. The fatty acids C14:0, C16:0 and 3-OHC14:0 were common to all LPS preparations. By SDS-PAGE, Bp-LPS had two bands of low molecular mass, and Bpp- and Bbs-LPS showed a low molecular mass band together with ladder bands of high molecular mass. Immunological assays demonstrated that Bp-LPS reacted with antisera prepared from Bp and Bpp; Bpp-LPS reacted with antisera against Bpp and Bbs, and Bbs-LPS reacted with antisera against any of the three species. Bp-LPS showed biological activities comparable to those of E. coli LPS in terms of lethal toxicity, pyrogenicity, mitogenicity, macrophage activation, and induction of tumor necrosis factor. All activities of Bpp-LPS, except mitogenicity, were lower than those of E. coli LPS. Biological activities stronger or comparable to those of E. coli LPS were observed for Bbs-LPS.
对从百日咳博德特氏菌（Bp）、副百日咳博德特氏菌（Bpp）和支气管败血博德特氏菌（Bbs）中分离出的脂多糖（LPS）进行了化学成分、分子异质性以及免疫和生物学特性分析。所有LPS均含有庚糖、2-酮-3-脱氧辛酸（KDO）、葡糖胺、糖醛酸、磷酸盐和脂肪酸。脂肪酸C14:0、C16:0和3-羟基C14:0在所有LPS制剂中都很常见。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE），Bp-LPS有两条低分子量条带，而Bpp-LPS和Bbs-LPS显示出一条低分子量条带以及高分子量的梯形条带。免疫分析表明，Bp-LPS与用Bp和Bpp制备的抗血清发生反应；Bpp-LPS与抗Bpp和Bbs的抗血清发生反应，Bbs-LPS与针对这三种细菌中任何一种的抗血清发生反应。在致死毒性、致热原性、促有丝分裂性、巨噬细胞活化和肿瘤坏死因子诱导方面，Bp-LPS显示出与大肠杆菌LPS相当的生物学活性。Bpp-LPS除促有丝分裂性外的所有活性均低于大肠杆菌LPS。观察到Bbs-LPS具有比大肠杆菌LPS更强或与之相当的生物学活性。
