Fry N K, Rowbotham T J, Saunders N A, Embley T M
Molecular Biology Unit, Central Public Health Laboratory, London, U.K.
FEMS Microbiol Lett. 1991 Oct 1;67(2):165-8. doi: 10.1016/0378-1097(91)90348-e.
DNA coding for the 16S rRNA of an intracellular bacterium was directly amplified from lysed cells of a host amoebae using the polymerase chain reaction and primers specific for eubacteria. The amoebae had been used to recover an uncultured bacterium observed in the sputum of a patient with pneumonia. The amplified DNA was sequenced directly and compared with published 16S rRNA sequences. The analysis revealed that the intracellular bacterium is a member of the genus Legionella and that it is different from species, including L. pneumophila, for which 16S ribosomal RNA sequence data are available.
利用聚合酶链反应和针对真细菌的引物，从宿主变形虫的裂解细胞中直接扩增出一种细胞内细菌16S rRNA的DNA编码。这些变形虫曾用于培养从一名肺炎患者痰液中观察到的未培养细菌。对扩增出的DNA直接进行测序，并与已发表的16S rRNA序列进行比较。分析表明，这种细胞内细菌是军团菌属的一个成员，并且与包括嗜肺军团菌在内的、已有16S核糖体RNA序列数据的物种不同。
