用聚合酶链反应法检测冷却塔水中的军团菌属。
Detection of Legionella spp. in cooling tower water by the polymerase chain reaction method.
作者信息
Koide M, Saito A, Kusano N, Higa F
机构信息
First Department of Internal Medicine, Faculty of Medicine, University of the Ryukyus, Okinawa, Japan.
出版信息
Appl Environ Microbiol. 1993 Jun;59(6):1943-6. doi: 10.1128/aem.59.6.1943-1946.1993.
The presence of Legionella spp. in cooling tower water was investigated by using the polymerase chain reaction. Total Legionella spp. detection was performed with 20-mer 5S rRNA complementary DNA sequence primers, and specific Legionella pneumophila detection was performed with 20-mer and then 21-mer macrophage infectivity potentiator gene sequence primers. Of 27 cooling tower water samples, 25 were positive for Legionella spp., and 14 of these contained L. pneumophila.
采用聚合酶链反应对冷却塔水中军团菌属的存在情况进行了调查。使用20聚体5S rRNA互补DNA序列引物进行军团菌属的总体检测，使用20聚体然后是21聚体巨噬细胞感染增强基因序列引物进行嗜肺军团菌的特异性检测。在27份冷却塔水样中，25份军团菌属检测呈阳性，其中14份含有嗜肺军团菌。
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