Düzgün Nurşen, Sahin Mehmet, Genç Yasemin, Tutkak Hüseyin
Department of Clinical Immunology and Rheumatology, Ankara University Faculty of Medicine, Samanpazari 06100, Ankara, Turkey.
Ann N Y Acad Sci. 2007 Aug;1109:421-8. doi: 10.1196/annals.1398.048.
The aim of this study is to investigate the prevalence of antinucleosome antibody in systemic lupus erythematosus (SLE) and their association with disease activity and renal involvement. The study included 131 patients with SLE, 74 rheumatoid arthritis, 26 systemic sclerosis, and 50 healthy individuals. Antinucleosome antibody and anti-dsDNA antibody were measured by an enzyme-linked immunosorbent assay (ELISA). Antinuclear antibody was tested by immunofluorescence using HEp-2 cells. Out of 131 SLE patients, 72 (54.9%) were seropositive for antinucleosome antibody, which was significantly higher than only 3 of 74 (4%) patients with rheumatoid arthritis (chi(2) = 52.82, P < 0.001); none of the patients with systemic sclerosis and 50 healthy individuals were seropositive. The sensitivity and specificity of antinucleosome antibodies in SLE were 83.6% and 70%, respectively. Fifty-one (38.9%) of SLE patients had renal involvement. Among these patients, the rate of antinucleosome positivity and anti-dsDNA were 74.5% and 78.4%, respectively. Antinucleosome antibodies were found to be 31.4% positive in SLE patients lacking anti-dsDNA antibody. Antinucleosome antibodies significantly correlated with disease activity (r = 0.428, P < 0.001) and anti-dsDNA (r = 0518, P < 0.001). The positivity of antinucleosome antibodies was significantly higher in patients with renal disease than the subjects without renal disease (chi(2) = 12.89, P < 0.001). The results of our study have revealed that in SLE patients, antinucleosome antibody could be a useful parameter for the assessment of disease activity or renal involvement.
本研究旨在调查系统性红斑狼疮（SLE）中抗核小体抗体的患病率及其与疾病活动度和肾脏受累的关系。该研究纳入了131例SLE患者、74例类风湿关节炎患者、26例系统性硬化症患者和50名健康个体。采用酶联免疫吸附测定（ELISA）检测抗核小体抗体和抗双链DNA抗体。使用HEp-2细胞通过免疫荧光法检测抗核抗体。在131例SLE患者中，72例（54.9%）抗核小体抗体血清学阳性，这显著高于74例类风湿关节炎患者中仅3例（4%）阳性（χ² = 52.82，P < 0.001）；系统性硬化症患者和50名健康个体中均无血清学阳性者。SLE中抗核小体抗体的敏感性和特异性分别为83.6%和70%。51例（38.9%）SLE患者有肾脏受累。在这些患者中，抗核小体阳性率和抗双链DNA阳性率分别为74.5%和78.4%。在缺乏抗双链DNA抗体的SLE患者中，抗核小体抗体阳性率为31.4%。抗核小体抗体与疾病活动度显著相关（r = 0.428，P < 0.001），与抗双链DNA也显著相关（r = 0.518，P < 0.001）。有肾脏疾病的患者抗核小体抗体阳性率显著高于无肾脏疾病的受试者（χ² = 12.89，P < 0.001）。我们的研究结果表明，在SLE患者中，抗核小体抗体可能是评估疾病活动度或肾脏受累的有用参数。
