Ahmad-Zadeh C, Allet B, Greenblatt J, Weil R
Proc Natl Acad Sci U S A. 1976 Apr;73(4):1097-101. doi: 10.1073/pnas.73.4.1097.
Simian-virus-40-specific T-antigen was isolated by immunoprecipitation. From other studies we have proof that the T-antigen described in this work is coded by the viral DNA. The molecular weight estimated from electrophoretic mobility in sodium dodecyl sulfate-polyacrylamide gels of T-antigen isolated from nonpermissive mouse cells in abortive infection is 86,000 and from permissive monkey cells in lytic infection is 82,000. The 86 kilodalton T-antigen is readily converted in vitro into an 82 kilodalton form by incubation with extracts from permissive monkey cells but not with extracts from nonpermissive mouse or hamster cells. This and the results of fingerprinting analysis of tryptic peptides suggest that T-antigen may be processed in permissive cells.
通过免疫沉淀法分离出了猿猴病毒40特异性T抗原。从其他研究中我们证实，本研究中描述的T抗原由病毒DNA编码。在流产感染中，从不允许感染的小鼠细胞中分离出的T抗原，经十二烷基硫酸钠-聚丙烯酰胺凝胶电泳迁移率估算的分子量为86,000；在裂解感染中，从允许感染的猴细胞中分离出的T抗原分子量为82,000。86千道尔顿的T抗原通过与允许感染的猴细胞提取物孵育，可在体外轻易转化为82千道尔顿的形式，但与不允许感染的小鼠或仓鼠细胞提取物孵育则不会转化。这以及胰蛋白酶肽指纹分析结果表明，T抗原可能在允许感染的细胞中进行加工处理。
Zotero 插件
