Matter J M, Weil R
Nucleic Acids Res. 1982 Dec 11;10(23):7643-55. doi: 10.1093/nar/10.23.7643.
In polyoma-infected mouse kidney cell cultures 5S RNA synthesis began to increase around 16 h, i.e. 7-9 h after the onset of polyoma T-antigen synthesis. The rate of polyoma-induced 5S RNA synthesis reached a maximum plateau around 25 h when it was 1.8-2.0 times higher than in mock-infected parallel cultures. Stimulation of 5S RNA synthesis in vivo thus coincided in time with the increase in total cellular RNA and protein. Cell-free extracts (S100) prepared at 15 h from mock-(S100-M) or polyoma-infected (S100-Py) mouse kidney cell cultures were indistinguishable with respect to protein concentration and 5S RNA synthesis, using a cloned somatic Xenopus borealis 5S gene as template. S100-Py extracted 25 h after infection contained 30% more protein and synthesized 1.5-2.0 times more 5S RNA than S100-M. Complete removal of the polyoma T-antigens from S100-Py by 3 cycles of immunoprecipitation with hamster anti-T serum remained without effect on stimulated 5S RNA synthesis. However, a linear relationship between 5S RNA synthesis and protein concentration of S100-M and S100-Py was observed.
在多瘤病毒感染的小鼠肾细胞培养物中，5S RNA合成在约16小时左右开始增加，即多瘤病毒T抗原合成开始后7 - 9小时。多瘤病毒诱导的5S RNA合成速率在约25小时达到最大平台期，此时比模拟感染的平行培养物高1.8 - 2.0倍。因此，体内5S RNA合成的刺激在时间上与总细胞RNA和蛋白质的增加相吻合。以克隆的非洲爪蟾体细胞5S基因作为模板，在15小时从模拟感染（S100 - M）或多瘤病毒感染（S100 - Py）的小鼠肾细胞培养物中制备的无细胞提取物（S100），在蛋白质浓度和5S RNA合成方面没有区别。感染后25小时提取的S100 - Py比S100 - M含有多30%的蛋白质，并且合成的5S RNA多1.5 - 2.0倍。用仓鼠抗T血清通过3轮免疫沉淀从S100 - Py中完全去除多瘤病毒T抗原，对受刺激的5S RNA合成没有影响。然而，观察到S100 - M和S100 - Py的5S RNA合成与蛋白质浓度之间存在线性关系。
