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转化细胞DNA中的整合猿猴病毒40序列：使用限制性内切酶进行分析。

Integrated simian virus 40 sequences in transformed cell DNA: analysis using restriction endonucleases.

作者信息

Ketner G, Kelly T J

出版信息

Proc Natl Acad Sci U S A. 1976 Apr;73(4):1102-6. doi: 10.1073/pnas.73.4.1102.

DOI:10.1073/pnas.73.4.1102
PMID:177975
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC430208/
Abstract

The DNAs from five independent simian virus 40 (SV40) transformants of the BALB/c3T3 mouse cell line were digested with either the HpaII or the BamHI restriction endonuclease and the resulting fragments were fractionated by gel electrophoresis. The DNA fragments were denatured in situ in the gel and transferred to a membrane filter. Fragments containing viral DNA were detected by hybridization with high specific activity 32P-labeled SV40 complementary RNA (cRNA) synthesized in vitro. Each of the lines yielded a small number of fragments containing SV40 DNA and the fragments from each line were different. This observation shows that the structure of the integrated SV40 DNA and/or its location in the host DNA are different in each line.

摘要

用HpaII或BamHI限制性内切酶消化来自BALB/c3T3小鼠细胞系的五个独立猿猴病毒40（SV40）转化体的DNA，所得片段通过凝胶电泳进行分离。DNA片段在凝胶中原位变性，然后转移到膜滤器上。通过与体外合成的高比活性32P标记的SV40互补RNA（cRNA）杂交来检测含有病毒DNA的片段。每个细胞系都产生了少量含有SV40 DNA的片段，并且每个细胞系的片段都不同。这一观察结果表明，整合的SV40 DNA的结构和/或其在宿主DNA中的位置在每个细胞系中都不同。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd85/430208/9aa543ecd8e3/pnas00034-0131-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd85/430208/8d63559d5132/pnas00034-0130-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd85/430208/0bc69193e0cf/pnas00034-0130-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd85/430208/9aa543ecd8e3/pnas00034-0131-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd85/430208/8d63559d5132/pnas00034-0130-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd85/430208/0bc69193e0cf/pnas00034-0130-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bd85/430208/9aa543ecd8e3/pnas00034-0131-a.jpg

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