Tereba A, Astrin S M
J Virol. 1980 Sep;35(3):888-94. doi: 10.1128/JVI.35.3.888-894.1980.
The distribution of 12 endogenous retrovirus loci from 13 White Leghorn flocks of diverse geographical origin was examined, using a total of 508 birds. Only one locus, designated ev-1, was observed to be almost universally present (506 of 508 birds) and thus predominantly homozygous in this breed of chicken. By contrast, 11 other viral loci were observed at much lower frequencies, 3 to 30%, and were occasionally restricted to individual flocks. To further characterize the commonly occurring ev-1 viral locus and to provide a foundation for determining the mechanism by which these 12 endogenous retrovirus loci were generated, a new technique of indirect in situ hybridization was used to localize the chromosomal position of ev-1. By using a combination of specific probes, the position of ev-1 was localized to a small region on the long arm of chromosome 1. This research represents oen of the first instances in which a defined segment of nonreiterated DNA coding for a known set of genes has been localized to a specific region on a vertebrate chromosome by in situ hybridization.
对来自13个不同地理来源的白来航鸡群的12个内源性逆转录病毒位点的分布进行了检测，共检测了508只鸡。仅观察到一个位点，命名为ev-1，几乎普遍存在（508只鸡中有506只），因此在这种品种的鸡中主要是纯合的。相比之下，其他11个病毒位点的出现频率要低得多，为3%至30%，并且偶尔局限于个别鸡群。为了进一步表征常见的ev-1病毒位点，并为确定这12个内源性逆转录病毒位点的产生机制提供基础，采用了一种间接原位杂交新技术来定位ev-1的染色体位置。通过使用特定探针的组合，ev-1的位置被定位到1号染色体长臂上的一个小区域。这项研究代表了首次通过原位杂交将编码一组已知基因的明确非重复DNA片段定位到脊椎动物染色体特定区域的实例之一。
