Varmus H E, Vogt P K, Bishop J M
Proc Natl Acad Sci U S A. 1973 Nov;70(11):3067-71. doi: 10.1073/pnas.70.11.3067.
A relatively simple but stringent technique was developed to detect the integration of virus-specific DNA into the genomes of higher organisms. In both permissive (duck) and nonpermissive (mammalian) cells which normally contain no nucleotide sequences specific for Rous sarcoma virus, transformation by the virus results in the appearance of DNA specific for Rous sarcoma virus covalently integrated into strands of host-cell DNA containing reiterated sequences. Early after infection of mouse or duck cells by Rous sarcoma virus, unintegrated DNA specific for the virus can be demonstrated.
人们开发出了一种相对简单但严格的技术,用于检测病毒特异性DNA整合到高等生物基因组中的情况。在通常不含有罗氏肉瘤病毒特异性核苷酸序列的允许性(鸭)细胞和非允许性(哺乳动物)细胞中,该病毒诱导的转化都会导致罗氏肉瘤病毒特异性DNA出现,这些DNA共价整合到含有重复序列的宿主细胞DNA链中。在罗氏肉瘤病毒感染小鼠或鸭细胞后不久,就能检测到病毒的未整合特异性DNA。