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无脊椎动物金属硫蛋白的纯化

Purification of invertebrate metallothioneins.

作者信息

Roesijadi G, Fowler B A

出版信息

Methods Enzymol. 1991;205:263-73. doi: 10.1016/0076-6879(91)05106-6.

DOI:10.1016/0076-6879(91)05106-6
PMID:1779786
Abstract

Purification procedures that appear to be generally applicable to invertebrate MTs have only recently been developed and are described here. Thus far, few invertebrate MTs have been purified and characterized, although proteins that exhibit similarities to MT have been identified in numerous invertebrate species. A greater understanding of MTs of this group, which comprises most of the animals in the animal kingdom, will provide the basis for increased understanding of the evolution of this ubiquitous protein. Additionally, specific invertebrate species may provide useful models for probing MT function and contribute to a greater understanding of the biological role of MT.

摘要

直到最近才开发出似乎普遍适用于无脊椎动物金属硫蛋白(MTs)的纯化程序,并在此进行描述。到目前为止,虽然在许多无脊椎动物物种中已鉴定出与MT具有相似性的蛋白质,但很少有无脊椎动物MTs被纯化和表征。对这一包含动物界大多数动物的类群的MTs有更深入的了解,将为增进对这种普遍存在的蛋白质进化的理解提供基础。此外,特定的无脊椎动物物种可能为探究MT功能提供有用的模型,并有助于更深入地理解MT的生物学作用。

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1
Purification of invertebrate metallothioneins.无脊椎动物金属硫蛋白的纯化
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2
Gel permeation, ion-exchange and reversed-phase columns for separation of metallothioneins by high-performance liquid chromatography-atomic absorption spectrophotometry.
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