人乳头瘤病毒DNA：物理图谱与遗传异质性
Human papilloma virus DNA: physical mapping and genetic heterogeneity.
作者信息
Gissmann L, zur Hausen H
出版信息
Proc Natl Acad Sci U S A. 1976 Apr;73(4):1310-3. doi: 10.1073/pnas.73.4.1310.
The molecular weight of three preparations of human papilloma virus DNA derived from different plantar warts was determined by agarose gel electrophoresis or electron microscopic contour length measurement. It was found to amount to approximately 4.9 X 10(6). Analysis of this DNA after sequential digestion by four different restriction endonucleases (EcoRI, Bam, Hind II, and Hind III) permitted physical mapping of the cleavage sites. Two of the three DNA preparations revealed an identical cleavage pattern, whereas the third one contained two additional cleavage sites.
通过琼脂糖凝胶电泳或电子显微镜轮廓长度测量法，测定了从不同足底疣中提取的三种人乳头瘤病毒DNA制剂的分子量。结果发现其分子量约为4.9×10⁶ 。用四种不同的限制性内切酶（EcoRI、Bam、Hind II和Hind III）对该DNA进行顺序酶切后分析，得以绘制出切割位点的物理图谱。三种DNA制剂中的两种呈现出相同的切割图谱，而第三种制剂含有另外两个切割位点。
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