Favre M, Orth G, Croissant O, Yaniv M
J Virol. 1977 Mar;21(3):1210-4. doi: 10.1128/JVI.21.3.1210-1214.1977.
The DNA of human papillomavirus (HPV) obtained from a pool of plantar warts is cleaved by bacillus amyloliquefaciens (BamI) and Haemophilus parainfluenzae (HpaII) restriction endonucleases at one and four specific sites, respectively. These sites were localized on the previously established cleavage map of HPV DNA, using the Hind, HindIII, HpaI, and EcoRI endonuclease restriction sites as reference. The four HpaII sites were mapped, clockwise, at 1.4, 41.1, 44.3, and 52.8% of the genome length from the unique BamI cleavage site taken as point zero. The HpaII site mapped at 1.4% of the genome length was absent in 40 to 50% of the molecules, thus showing a genetic heterogeneity of HPV DNA.
从一组足底疣中获取的人乳头瘤病毒（HPV）的DNA，分别被解淀粉芽孢杆菌（BamI）和副流感嗜血杆菌（HpaII）限制性内切酶在一个和四个特定位点切割。以Hind、HindIII、HpaI和EcoRI内切酶的限制性位点作为参考，这些位点被定位在先前建立的HPV DNA切割图谱上。四个HpaII位点按顺时针方向，从唯一的BamI切割位点（设为零点）起，分别位于基因组长度的1.4%、41.1%、44.3%和52.8%处。位于基因组长度1.4%处的HpaII位点在40%至50%的分子中不存在，因此显示出HPV DNA的遗传异质性。
