Palomar J, Viñas M
Laboratory of Microbiology, School of Pharmacy, University of Barcelona, Spain.
Microbiologia. 1996 Sep;12(3):435-8.
Serratia marcescens is an enterobacterium that exhibits very low efficiency of transformation. According to previous work, neither the bacterium restriction system nor its nuclease production accounts for this low efficiency. Differences in the efficiency of transformation from plasmid DNA were found in wild type of S. marcescens and their O-deficient spontaneous mutant strains. This phenomenon seems to be independent of plasmid size. When electroporation was used, the survival of O-mutants was much lower than those of their parental strains, but the frequencies of transformation among survivors were much higher. This suggests that the presence of the O-antigen is responsible for the low transformation frequencies observed.
粘质沙雷氏菌是一种转化效率极低的肠道细菌。根据之前的研究，该细菌的限制系统及其核酸酶产生均不能解释这种低效率。在粘质沙雷氏菌的野生型及其O抗原缺陷自发突变株中发现了从质粒DNA转化效率的差异。这种现象似乎与质粒大小无关。当使用电穿孔时，O突变体的存活率远低于其亲本菌株，但存活者中的转化频率要高得多。这表明O抗原的存在是观察到低转化频率的原因。
