Joho M, Tarumi K, Inouhe M, Tohoyama H, Murayama T
Department of Biology, Faculty of Science, Ehime University, Matsuyama, Japan.
Microbios. 1991;67(272-273):177-86.
A mutant strain of Saccharomyces cerevisiae (NR 6), which can be associated with a reduction in the accumulation of Mg2+ has been isolated. This mutant strain displays resistance to both Ni2+ and Co2+, but not, however, towards Cu2+, Cd2+, Mn2+, Zn2+ and Cr2O7(2-). Both Co2+ and Ni2+ uptake by the mutant strain is less than by the wild type (CMR-50). The inhibitory effects of Ni2+ and Co2+ on the growth of both strains NR-6 and CMR-50 can be cancelled by increasing the concentrations of Mg2+ in the medium and to a lower extent by the addition of Ca2+ which results in a decreased uptake of these metals. It therefore appears that the resistant mechanisms of this mutant strain NR-6 is due to a reduction in the uptake of Co2+ and Ni2+ via a Mg2+ transport system.
已分离出一种酿酒酵母突变株（NR 6），其可能与Mg2+积累减少有关。该突变株对Ni2+和Co2+均具有抗性，但对Cu2+、Cd2+、Mn2+、Zn2+和Cr2O7(2-)不具有抗性。突变株对Co2+和Ni2+的摄取均少于野生型（CMR - 50）。通过增加培养基中Mg2+的浓度，可消除Ni2+和Co2+对NR - 6和CMR - 50这两种菌株生长的抑制作用，而添加Ca2+在较低程度上也可消除这种抑制作用，这会导致这些金属的摄取减少。因此，该突变株NR - 6的抗性机制似乎是由于通过Mg2+转运系统对Co2+和Ni2+的摄取减少所致。
