Zhu Mei-fen, Liu Xiang-qian, Ju-hee Oh, Chang-soo Yook, Je-Hyun Lee
Department of Oriental Pharmaceutical Sciences, College of Phamacy, Kyung Hee University, Seoul 130701, Korea.
Zhongguo Zhong Yao Za Zhi. 2007 Jun;32(12):1155-7.
To study on effect of concentration of catalpol and 5-hydroxy methyl-2-furaldehyde (5-HMF) from Rehmanniae Radix at various processing.
The Rehmanniae Radix was dried and prepared from the steaming process with 10% ethanol, 50% ethanol at 90 degrees C and 100 degrees C each other. And the changes of catalpol and 5-HMF was determinated. The extraction of 5-HMF and catalpol was sonicated in 30% methanol for 2 h. The analysis of 5-HMF and catalpol was conducted by HPLC with reversed-phase C-18 column and detected under UV 284 nm, 204 nm. Elution was carried out at 1.0 mL min(-1) with 3% acetonitrile.
From this analysis, we found out that the content of catalpol was decreased with the number of processing times, and content of 5-HMF was increased with the number of processing times at various processing. The temperature and concentration of ethanol can effect on content of catalpol and 5-HMF at processing. The Cooked Rehmanniae Radix processed at 100 degrees C, 10% ethanol is best. And the content of 5-HMF processed for more than 7 times was accorded with standard of Korea phamcopoetia.
Analyze the effect of concentration of catalpol and 5-HMF from Rehmanniae Radix at various processing, and provide the foundation for further study.
研究不同炮制方法对地黄中梓醇和5-羟甲基糠醛（5-HMF）含量的影响。
地黄经干燥后，分别用10%乙醇、50%乙醇在90℃和100℃进行蒸制处理。测定梓醇和5-HMF的含量变化。5-HMF和梓醇的提取采用30%甲醇超声处理2小时。采用反相C-18柱，在紫外284nm、204nm波长下，通过高效液相色谱法分析5-HMF和梓醇。以3%乙腈为流动相，流速为1.0 mL min(-1)进行洗脱。
分析发现，在不同炮制过程中，梓醇含量随炮制次数增加而降低，5-HMF含量随炮制次数增加而升高。乙醇的温度和浓度对炮制过程中梓醇和5-HMF的含量有影响。100℃、10%乙醇炮制的熟地黄最佳。5-HMF炮制7次以上的含量符合韩国药典标准。
分析不同炮制方法对地黄中梓醇和5-HMF含量的影响，为进一步研究提供依据。
