Huang Li-Ting, Sun Shao-Ping, Zheng Ying
State Key Laboratory of Quality Research in Chinese Medicine/Institute of Chinese Medical Sciences, University of Macau, Macao SAR 999078, China.
Zhong Yao Cai. 2012 Aug;35(8):1318-22.
To establish a method for simultaneously quantification of the Catalpol and Acteoside from Rehmanniae Radix and optimize the extraction process from Rehmanniae Radix by response surface methodology (RSM) using central composite design (CCD).
The effect of ethanol concentration and the solvent-material ratio of the extraction were investigated on the overall extraction efficiency. Design Expert was used for regression analysis and determination coefficient of the regression equation.
A good linear relationship was obtained from 0.047 - 3.00 microg and 0.016 - 1.00 microg for Catalpol and Acteoside, respectively. The optimal condition of extraction process was as follows: raw material was extracted with 19.3 mL/g solvent-material ratio of 38.0% ethanol. The R-Squared of binomial fitting of the complex model was 0.7498 (P < 0.05). The experimental values were accordance with values obtained from model prediction.
The accuracy and precision of the developed HPLC protocol is adequate for simultaneous quantification of Catalpol and Acteoside in Rehmanniae Radix. The optimized extraction condition could be obtained by RSM using CCD.
建立同时定量生地黄中梓醇和毛蕊花糖苷的方法，并采用中心复合设计（CCD）的响应面法（RSM）优化生地黄的提取工艺。
考察乙醇浓度和提取溶剂与物料比对整体提取效率的影响。使用Design Expert进行回归分析和回归方程的决定系数分析。
梓醇和毛蕊花糖苷分别在0.047 - 3.00 μg和0.016 - 1.00 μg范围内呈良好的线性关系。提取工艺的最佳条件如下：以19.3 mL/g的溶剂与物料比、38.0%的乙醇提取原料。复合模型的二项式拟合的R平方为0.7498（P < 0.05）。实验值与模型预测值相符。
所建立的高效液相色谱法的准确度和精密度足以同时定量生地黄中的梓醇和毛蕊花糖苷。采用CCD的RSM可获得优化的提取条件。
