Yang Yun, Xu Min, Feng Yun-xia, He Hai-hua
Henan College of Traditional Chinese Medicine, Zhengzhou, China.
Zhong Yao Cai. 2008 Jan;31(1):17-9.
By finding the changing regularity of 5-Hydroxymethyl furfuraldehyde (5-HMF) content in Polygonatum for different time, this provides basis for the technology of processing Polygonatum and quality standards of its prepared pieces.
The testing solution was acquired by ultrasonic extraction, HPLC was used to determine the contents. The chromatographic conditions: Agilent HC-C18 column (5 microm, 250 mm x 4.6 mm). The mobile phase: methanol-water (15:85) at the flow rate of 1 ml/min. The determination wave length was 284 nm.
All kinds of processed Polygonatum had 5-Hydroxymethyl furfuraldethyde in different degrees, contents in Polygonatum kept relatively stable before 30 hours, but contents rose rapidly after 30 hours, contents decreased as continuing heating.
The result can provide useful references to quality control and standard porcessing condition of Polygonatum.
通过研究不同时间黄精中5-羟甲基糠醛（5-HMF）含量的变化规律，为黄精炮制工艺及饮片质量标准提供依据。
采用超声提取法制备供试品溶液，用高效液相色谱法测定含量。色谱条件：Agilent HC-C18柱（5μm，250mm×4.6mm）。流动相：甲醇-水（15:85），流速为1ml/min。测定波长为284nm。
各种炮制黄精均含有不同程度的5-羟甲基糠醛，30小时前黄精中5-HMF含量相对稳定，但30小时后含量迅速上升，继续加热含量下降。
该结果可为黄精的质量控制和规范炮制条件提供有益参考。
