Ma Ming, Shang Xiao-ya, Wang Su-juan, Li Shuai, Yang Yong-chun, Shi Jian-gong
Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine Ministry of Education, Institute of Materia Medica, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100050, China.
Zhongguo Zhong Yao Za Zhi. 2007 Jun;32(12):1175-9.
To investigate the chemical constituents of the brach of Macaranga adenantha, and evaluate their TNF-alpha inhibitory activity.
The chemical conshifuents were isolated and purified by chromatographic methods. Structures of the compounds were identified by spectroscopic methods. The TNF-alpha secretion inhibitory activity of the mouse peritoneal macrophages was evaluated by MTT methods.
Ten compounds were isolated and their structures were identified as: cleomiscosin A (1), cleomiscosin B (2), ellagic acid 4-O-alpha-D-rhamnopyranside (3), ellagic acid 4-O-beta-D-xylopyranoside (4), vanillic acid (5), (24R) -stigmast-4-en-3-one (6), (24R) -stigmast-3, 6-dione (7), (24R) -6beta-hydroxy-stigmast-4-en-3-one (8), daucosterol (9), beta-sitosteryl glucoside-6'-O-heptadecoicate (10). At a concentration of 10 micromol x L(-1), compounds 1, 3 and 4 showed inhibitory activity to TNF-alpha secretion of the mouse peritoneal macrophages with the inhibitory rates of 57.0%, 64.4%, and 57. 4%, respectively.
All compounds were isolated from genus Macaranga for the first time. Compounds 1, 3, and 4 were active against TNF-alpha secretion of the mouse peritoneal macrophages.
研究腺苞血桐枝条的化学成分，并评估其对肿瘤坏死因子-α（TNF-α）的抑制活性。
采用色谱法分离纯化化学成分。通过光谱法鉴定化合物结构。采用MTT法评估小鼠腹腔巨噬细胞TNF-α分泌抑制活性。
分离得到10个化合物，其结构鉴定为：刺山柑辛素A（1）、刺山柑辛素B（2）、鞣花酸4-O-α-D-鼠李吡喃糖苷（3）、鞣花酸4-O-β-D-木吡喃糖苷（4）、香草酸（5）、（24R）-豆甾-4-烯-3-酮（6）、（24R）-豆甾-3,6-二酮（7）、（24R）-6β-羟基-豆甾-4-烯-3-酮（8）、胡萝卜苷（9）、β-谷甾醇葡萄糖苷-6'-O-十七烷酸酯（10）。在浓度为10 μmol·L⁻¹时，化合物1、3和4对小鼠腹腔巨噬细胞TNF-α分泌具有抑制活性，抑制率分别为57.0%、64.4%和57.4%。
所有化合物均首次从血桐属植物中分离得到。化合物1、3和4对小鼠腹腔巨噬细胞TNF-α分泌具有活性。
