肾移植受者中BK病毒的定量聚合酶链反应检测及感染监测

BKV QPCR detection and infection monitoring in renal transplant recipients.

作者信息

Mischitelli Monica, Fioriti Daniela, Anzivino Elena, Bellizzi Anna, Ferretti Giancarlo, Gussman Norberto, Mitterhofer Anna Paola, Tinti Francesca, Barile Mario, Dal Maso Maurizio, Chiarini Fernanda, Pietropaolo Valeria

机构信息

Department of Public Health Sciences, University La Sapienza Rome, Italy.

出版信息

New Microbiol. 2007 Jul;30(3):271-4.

PMID:17802907

Abstract

BKV associated nephropathy (BKVAN) is a cause of renal dysfunction and loss of the graft in transplants. Viral primary infection is usually inapparent and then BKV establishes latency in kidneys. Reactivation occurs in immunocompromised conditions in renal transplant recipients who can develop a subclinical nephritis and eventually a BKV-associated interstitial nephritis or a BKVAN. In this study, we searched for BKV copies in urine and plasma of renal transplants by quantitative assay (QPCR). Results showed that in several patients clearance of viremia is associated with persistent viruria, suggesting that both specimens are necessary to correctly monitor a BKVAN.

摘要

BK病毒相关性肾病（BKVAN）是导致移植肾功能障碍和移植物丧失的原因之一。病毒的初次感染通常不明显，随后BK病毒在肾脏中建立潜伏状态。在肾移植受者免疫功能低下的情况下，病毒会重新激活，可发展为亚临床肾炎，最终发展为BK病毒相关性间质性肾炎或BKVAN。在本研究中，我们通过定量分析（QPCR）检测肾移植受者尿液和血浆中的BK病毒拷贝数。结果显示，在一些患者中，病毒血症的清除与持续性病毒尿有关，这表明两种标本对于正确监测BKVAN都是必要的。