Wang Gang, Szeto Cheuk-Chun
Department of Medicine & Therapeutics, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, Hong Kong, China.
Nephrology (Carlton). 2007 Oct;12(5):494-9. doi: 10.1111/j.1440-1797.2007.00836.x.
Urinalysis has been used extensively in clinical practice to aid in the diagnosis of various renal diseases. With the advances in technology of molecular biology, gene expression and proteomic studies are now possible for urinary sediment. In this review article, we focus on the quantification of messenger RNA expression in urinary sediment by reverse-transcription and real-time quantitative polymerase chain reaction. Recently, this technique has been explored for three potential applications: (i) distinguishing the different causes of kidney disease; (ii) assessment of kidney disease activity, progression and response to therapy; and (iii) as a tool to study the pathogenesis. Although the method is simple and non-invasive, it requires further research to define the role in routine clinical practice. At this moment, the technique should only be considered experimental albeit promising.
尿液分析在临床实践中已被广泛用于辅助诊断各种肾脏疾病。随着分子生物学技术的进步，现在对尿沉渣进行基因表达和蛋白质组学研究成为可能。在这篇综述文章中，我们重点关注通过逆转录和实时定量聚合酶链反应对尿沉渣中信使核糖核酸表达进行定量。最近，这项技术已被探索用于三个潜在应用：（i）区分肾脏疾病的不同病因；（ii）评估肾脏疾病的活动、进展及对治疗的反应；（iii）作为研究发病机制的工具。尽管该方法简单且非侵入性，但仍需要进一步研究以明确其在常规临床实践中的作用。目前，该技术尽管前景广阔，但仍应仅被视为实验性技术。
