Gonçalves Silvana B, Dong Zhihong, Bramante Clovis M, Holland Graham R, Smith Anthony J, Nör Jacques E
Department of Cariology, Restorative Sciences and Endodontics, University of Michigan School of Dentistry, Ann Arbor, Michigan 48109-1078, USA.
J Endod. 2007 Jul;33(7):811-4. doi: 10.1016/j.joen.2007.03.012. Epub 2007 May 7.
Treatment of avulsed young permanent teeth aims to revascularize the dental pulp. The study of therapeutic strategies for avulsed teeth has been hindered by the scarcity of experimental models. The purpose of this work is to characterize two model systems to study dental pulp revascularization. Tooth slices from human third molars were prepared with a sterile diamond saw. The tooth slices were cultured in vitro for up to 7 days. Immunohistochemical staining with Factor VIII showed an increase in microvascular density in pulps treated with 50 ng/mL rhVEGF(165) as compared with untreated controls (p < 0.05). Alternatively, tooth slices were prepared and immediately implanted subcutaneously in immunodeficient mice. Pulp vitality and vascularization were confirmed by histological analysis and terminal deoxynucleotide transferase dUTP nick end labeling assays 7 days after implantation. The models presented here may be valuable in the assessment of angiogenesis-based therapeutic strategies for the dental pulp.
年轻恒牙脱位的治疗旨在使牙髓再血管化。实验模型的匮乏阻碍了对脱位牙治疗策略的研究。本研究的目的是对两种用于研究牙髓再血管化的模型系统进行表征。用无菌金刚石锯制备人第三磨牙的牙片。将牙片在体外培养长达7天。与未处理的对照组相比，用50 ng/mL rhVEGF(165)处理的牙髓中，VIII因子免疫组化染色显示微血管密度增加(p < 0.05)。另外，制备牙片并立即皮下植入免疫缺陷小鼠体内。植入7天后，通过组织学分析和末端脱氧核苷酸转移酶dUTP缺口末端标记试验确认牙髓活力和血管化。这里介绍的模型在评估基于血管生成的牙髓治疗策略方面可能具有重要价值。
