Riley Kasandra J-L, Maher L James
Department of Biochemistry and Molecular Biology, Mayo Clinic, College of Medicine, Rochester, MN 55905, USA.
RNA. 2007 Nov;13(11):1825-33. doi: 10.1261/rna.673407. Epub 2007 Sep 5.
The p53 tumor suppressor protein is typically considered to be a sequence-specific DNA-binding transcription factor. However, reports over the last 15 years have described RNA binding by p53 in a variety of contexts, suggesting the possibility of new p53 functions. It is clear that p53-RNA interactions are mediated by a nucleic acid-binding domain of p53 independent of the sequence-specific core domain responsible for DNA recognition. Reports disagree on several aspects of the putative RNA interaction, including sequence specificity and biological relevance. Here we review the history and recent advances in the study of p53-RNA interactions. We argue that p53-RNA interactions are sequence nonspecific and depend on incomplete post-translational modification of the p53 C-terminal domain when the protein is expressed in heterologous systems. It is unknown what fraction of p53 protein exists in a state competent for RNA binding in vivo. Thus, potential physiological roles of p53-RNA interactions remain mysterious.
p53肿瘤抑制蛋白通常被认为是一种序列特异性的DNA结合转录因子。然而，过去15年的报告描述了在多种情况下p53与RNA的结合，这暗示了p53具有新功能的可能性。很明显，p53与RNA的相互作用是由p53的一个核酸结合结构域介导的，该结构域独立于负责DNA识别的序列特异性核心结构域。关于假定的RNA相互作用的几个方面，报告存在分歧，包括序列特异性和生物学相关性。在这里，我们回顾了p53与RNA相互作用研究的历史和最新进展。我们认为，当该蛋白在异源系统中表达时，p53与RNA的相互作用是非序列特异性的，并且依赖于p53 C末端结构域不完全的翻译后修饰。目前尚不清楚在体内有多少比例的p53蛋白处于能够与RNA结合的状态。因此，p53与RNA相互作用的潜在生理作用仍然是个谜。
