Orstavik I, Figenschau K J, Haug K W, Ulstrup J C
Scand J Infect Dis. 1976;8(1):1-5.
A reovirus-like agent (rotavirus) was detected in 26 children (44%) when fecal specimens from 59 children with acute gastroenteritis were examined by electron microscopy. No rotavirus was detected in the feces of 49 children with other diseases. Sera from the acute and the convalescent phase from 40 children with acute gastroenteritis and from 18 other patients were examined for antibodies against a bovine rotavirus by an indirect fluorescent antibody test. 26 of the patients with gastroenteritis (65%) developed antibodies during their disease, whereas none of the other patients showed a rise in antibody titre. The specimens were submitted to the laboratory from hospitalized children during the period January 1973 through March 1975. Most of the cases of rotavirus gastroenteritis occurred during late autumn and early winter among children between 0.5 and 3 years of age. It is concluded that electron microscopy is a sensitive diagnostic technique during the acute phase of the disease, and that the serological test with bovine rotavirus antigen served as a useful diagnostic tool.
用电子显微镜检查59例急性胃肠炎患儿的粪便标本时，在26例患儿（44%）中检测到一种呼肠孤病毒样病原体（轮状病毒）。在49例患有其他疾病的患儿粪便中未检测到轮状病毒。采用间接荧光抗体试验检测了40例急性胃肠炎患儿以及18例其他患者急性期和恢复期血清中抗牛轮状病毒抗体。26例胃肠炎患者（65%）在患病期间产生了抗体，而其他患者均未出现抗体滴度升高。这些标本于1973年1月至1975年3月期间由住院患儿提交至实验室。大多数轮状病毒胃肠炎病例发生在晚秋和初冬，患儿年龄在0.5至3岁之间。得出的结论是，电子显微镜检查是疾病急性期的一种敏感诊断技术，而用牛轮状病毒抗原进行的血清学检测是一种有用的诊断工具。
