Holdaway M D, Kalmakoff J, Schroeder B A, Wright G C, Todd B A, Jennings L C
N Z Med J. 1982 Feb 24;95(702):110-2.
A method for measuring rotavirus antibody in human sera has been established using enzyme-linked immunosorbent assay (ELISA). A Simian strain of rotavirus (SA11) was used as the antigen. Serum eluted from dried blood spots on good quality chromatography paper was found suitable for analysis. Paired serum samples from children with gastroenteritis have shown a brisk antibody response in association with the presence of rotavirus in the faeces. Community studies indicate that although all older children and adults tested have detectable antibodies to rotavirus, there is a significant rise in the number of individuals with high titre antibody in the child bearing age group, after which the levels diminish. This finding suggests that repeated infections occur throughout childhood and early adult life.
已建立一种使用酶联免疫吸附测定（ELISA）法检测人血清中轮状病毒抗体的方法。使用一种猿猴轮状病毒株（SA11）作为抗原。发现从优质层析纸上的干血斑洗脱的血清适合进行分析。来自肠胃炎患儿的配对血清样本显示，与粪便中存在轮状病毒相关的抗体反应活跃。社区研究表明，尽管所有接受检测的大龄儿童和成人都有可检测到的轮状病毒抗体，但育龄儿童组中高滴度抗体个体的数量显著增加，之后抗体水平下降。这一发现表明，在整个儿童期和成年早期会反复发生感染。
