Science. 1983 Oct 7;222(4619):53-5. doi: 10.1126/science.222.4619.53.
Crab zoeae (Rhithropanopeus harrisii) were exposed during their development opment to a range of free cupric ion activities regulated in seawater by use of a copper chelate buffer system. Most cytosolic copper was found to be associated with metallothionein. Copper-thionein could be related to free cupric ion activity, and a shift in copper-thionein accumulation was correlated with inhibition of larval growth. These data reveal predictable relations between cupric ion activity in seawater and processes at the cellular and organismic levels.
在发育过程中，利用铜螯合缓冲系统将一系列游离铜离子活度调节到海水中，使三疣梭子蟹（Rhithropanopeus harrisii）幼体暴露于其中。大多数胞质铜与金属硫蛋白结合。铜-硫蛋白可能与游离铜离子活度有关，铜-硫蛋白的积累变化与幼虫生长抑制有关。这些数据揭示了海水中铜离子活度与细胞和机体水平过程之间可预测的关系。
