Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, University Park, NG7 2RD, Nottingham, UK.
Plant Cell Rep. 1985 May;4(3):164-7. doi: 10.1007/BF00571308.
Protoplasts of several wildLinum species were isolated enzymatically from roots, hypocotyls and cotyledons of seedlings, and also from theirin vitro grown shoots and cell suspension cultures. When cultured all these protoplasts divided to produce callus but only good plant regeneration capability was evident in the case ofLinum lewissii and to a much lesser extent forL. strictum. Only rhizogenesis was observed withL. alpinum, L. narbonense, L. grandiflorum andL. altaicum. The high plant regeneration capacity ofL. lewissii from protoplast -derived tissues ofin vitro shoots and cell suspension cultures makes this species an attractive experimental system for somatic genetic manipulation.
几种野生亚麻属物种的原生质体可通过酶从幼苗的根、下胚轴和子叶以及离体生长的茎和细胞悬浮培养物中分离出来。当所有这些原生质体培养时，都会分裂产生愈伤组织，但只有Lewissii 亚麻和在较小程度上的Strictum 亚麻表现出良好的植物再生能力。只有高山亚麻、纳博讷亚麻、大花亚麻和 Altaicum 亚麻观察到生根。Lewissii 亚麻从离体茎和细胞悬浮培养物的原生质体衍生组织中具有高的植物再生能力，这使其成为体细胞遗传操作的有吸引力的实验系统。
