Delgado-Baeza E, Giménez-Ribotta M, Miralles-Flores C, Nieto-Chaguaceda A, Santos-Alvarez I
Laboratorio de Microcirugía Experimental, Hospital La Paz, Madrid, España.
Acta Anat (Basel). 1991;142(2):132-7. doi: 10.1159/000147177.
This paper studies the participation of vessels in the canal morphogenesis. The proximal chondroepiphysis of the left tibia of 44 five-day-old rats was exposed and the vessels of the intercondylar fossae, near the attachment of cruciate ligaments, were cauterized. In addition to the vascular lesion this assay induced a perichondral lesion. However, the canal appeared and joined to the secondary ossification center. 36 tibiae of 4-day-old rats were removed under sterile conditions and cultured in a serum-free chemically defined medium. The cultures were carried out for as long as 15 days. A canal lumen structure was found on the first days of culture, and grew in depth to the central region of the chondroepiphysis during the culture. The secondary ossification center was not found. The vessels and mesenchymal cells observed in the control canal were not found in the culture. We suggest that the presence of vessels, perivascular cells or perichondrium does not appear to be necessary in canal morphogenesis.
本文研究血管在软骨管形态发生中的作用。暴露44只5日龄大鼠左胫骨的近端软骨骨骺，并烧灼髁间窝靠近交叉韧带附着处的血管。除了血管损伤外，该实验还诱发了软骨膜损伤。然而，软骨管出现并与二级骨化中心相连。在无菌条件下取出36只4日龄大鼠的胫骨，并在无血清化学限定培养基中培养。培养持续长达15天。在培养的第一天发现了软骨管腔结构，并在培养过程中向软骨骨骺的中央区域深入生长。未发现二级骨化中心。在对照软骨管中观察到的血管和间充质细胞在培养物中未发现。我们认为，在软骨管形态发生过程中，血管、血管周围细胞或软骨膜的存在似乎并非必要。
