Delgado-Baeza E, Nieto-Chaguaceda A, Miralles-Flores C, Santos-Alvarez I
Laboratorio de Microcirugía Experimental, Hospital La Paz, Madrid, España.
Acta Anat (Basel). 1992;145(2):143-8. doi: 10.1159/000147356.
This paper studies the influence of an antimitotic factor (puromycin) and a hormonal factor (thyroid hormone, TH) on canal growth. The tibiae of 15 six-day-old rats were cultured in a serum-free chemically defined medium. The cultures were carried out for as long as 6 days. Our results show: (1) canal growth is not dependent on perichondrium or chondro-epiphysis growth; (2) the canal is greater and has a complex pattern in a triiodothyronine (T3)-treated assay group; (3) round and multinucleated cells are more numerous in the T3-treated assay group than in the other groups. We hypothesize that the canal grows by a physiological phenomenon of programmed cell death and that it is stimulated by TH.
本文研究抗有丝分裂因子（嘌呤霉素）和激素因子（甲状腺激素，TH）对管生长的影响。将15只6日龄大鼠的胫骨在无血清化学限定培养基中培养。培养持续长达6天。我们的结果表明：（1）管生长不依赖于骨膜或软骨骨骺生长；（2）在三碘甲状腺原氨酸（T3）处理的试验组中管更大且具有复杂模式；（3）T3处理的试验组中圆形和多核细胞比其他组更多。我们假设管通过程序性细胞死亡的生理现象生长，并且它受到TH的刺激。
