Liu M, Okada S, Kawabata T
First Department of Pathology, Okayama University Medical School, Japan.
Acta Med Okayama. 1991 Dec;45(6):401-8. doi: 10.18926/AMO/32180.
Iron plays a critical role in the production of activated oxygen species and the activity of chelated iron in the biological system depends on the chemical forms of the chelators. In the present study, we used ferric nitrolotriacetate (Fe-NTA, molar ratio of iron to chelators = 1:3), ferric ethylenediaminetetraacetate (Fe-EDTA, 1:3 complex) and ferric Desferal (Fe-Des, 1:1.1 complex) to see their "free" iron content in aqueous solutions in vitro and in the serum obtained after a single intraperitoneal injection of the chelates to rats (7.5 mg of iron/kg). "Free" iron was measured by the bleomycin-assay system. When Fe-NTA was dissolved in water, "free" iron increased linearly with total iron concentration up to 10 microM, whereas Fe-EDTA and Fe-Des showed no "free" iron with corresponding iron concentrations. When these three ferric chelates were dissolved in normal rat serum, "free" iron in Fe-NTA increased abruptly between 40 microM and 60 microM iron concentrations, then increased slowly up to 100 microM. Fe-Des did not show any "free" iron at comparable iron concentrations. Fe-EDTA had an intermediate "free" iron level in the serum. Among the ferric chelate complexes, Fe-NTA showed a much faster increase of and a higher content of "free" iron in the serum than the other two complexes after a single injection of the chelates into rats.(ABSTRACT TRUNCATED AT 250 WORDS)
铁在活性氧的产生中起关键作用，生物系统中螯合铁的活性取决于螯合剂的化学形式。在本研究中，我们使用次氮基三乙酸铁（Fe-NTA，铁与螯合剂的摩尔比 = 1:3）、乙二胺四乙酸铁（Fe-EDTA，1:3 络合物）和去铁胺铁（Fe-Des，1:1.1 络合物）来观察它们在体外水溶液以及大鼠单次腹腔注射螯合物（7.5 毫克铁/千克）后获得的血清中的“游离”铁含量。“游离”铁通过博来霉素检测系统进行测量。当 Fe-NTA 溶解于水中时，“游离”铁随总铁浓度线性增加，直至 10 微摩尔，而 Fe-EDTA 和 Fe-Des 在相应铁浓度下未显示“游离”铁。当这三种三价铁螯合物溶解于正常大鼠血清中时，Fe-NTA 中的“游离”铁在铁浓度 40 微摩尔至 60 微摩尔之间急剧增加，然后缓慢增加至 100 微摩尔。在相当的铁浓度下，Fe-Des 未显示任何“游离”铁。Fe-EDTA 在血清中的“游离”铁水平处于中间。在三价铁螯合物中，单次给大鼠注射螯合物后，Fe-NTA 在血清中显示出比其他两种络合物更快的“游离”铁增加速度和更高的“游离”铁含量。（摘要截短于 250 字）
