Matsuura R
Acta Med Okayama. 1983 Oct;37(5):393-400. doi: 10.18926/AMO/32422.
A previous study has shown that a single injection of ferric nitrilotriacetate (Fe-NTA) produces hepatic parenchymal iron loading in rats. The present paper reports on iron uptake by rat liver and iron toxicity in the liver after a single injection of Fe-NTA (7.5 mg Fe/kg B.W.). Iron uptake was examined with 59Fe-NTA and Fe-[14C]-NTA. Thirty percent of the injected 59Fe was incorporated in the liver non-heme iron fraction at 3 h and retained for 240 h. Only 1% of the 14C injected as Fe-[14C]-NTA was taken up by the liver at 3 h. Gel filtration with a Sephadex G-25 column of the supernatant fraction of the liver obtained 3 h after the injection showed two peaks of 14C activity. One was eluted in the void volume, and the other corresponded to [14C]-NTA. The former had a molecular weight of 5,000-10,000 as determined with a Sephacryl S-300 column and also had 59Fe activity. The electron spin resonance spectra showed that the generation of a free radical in the liver was initiated within 1 h of the iron administration. The free radical generated in the serum by Fe-NTA was revealed to be superoxide by the spin trapping method. These results suggest that Fe-NTA transfers iron to transferrin in the serum and induces hepatic iron loading. Small amounts of the injected iron were taken up by the liver as Fe-NTA and generated superoxide which may have induced lipid peroxidation of the cellular membranes.
先前的一项研究表明，单次注射次氮基三乙酸铁（Fe-NTA）会使大鼠肝实质铁负荷增加。本文报道了单次注射Fe-NTA（7.5毫克铁/千克体重）后大鼠肝脏对铁的摄取及肝脏中的铁毒性。用59Fe-NTA和Fe-[14C]-NTA检测铁摄取情况。注射的59Fe中有30%在3小时时被肝脏非血红素铁组分摄取并保留240小时。作为Fe-[14C]-NTA注射的14C在3小时时只有1%被肝脏摄取。对注射后3小时获得的肝脏上清液组分用Sephadex G-25柱进行凝胶过滤，显示出两个14C活性峰。一个在空体积处洗脱，另一个对应于[14C]-NTA。用Sephacryl S-300柱测定，前者的分子量为5000 - 10000，并且也有59Fe活性。电子自旋共振光谱表明，在给予铁后1小时内肝脏中就开始产生自由基。通过自旋捕获法发现，Fe-NTA在血清中产生的自由基是超氧化物。这些结果表明，Fe-NTA将铁转移到血清中的转铁蛋白上并诱导肝脏铁负荷增加。少量注射的铁以Fe-NTA的形式被肝脏摄取并产生超氧化物，超氧化物可能诱导了细胞膜的脂质过氧化。
