Li K, Welsh M G
Department of Anatomy, Cell Biology and Neurosciences, School of Medicine, University of South Carolina, Columbia 29208.
Am J Anat. 1991 Dec;192(4):510-22. doi: 10.1002/aja.1001920415.
Postnatal development of S-Ag and GFAP immunoreactivity in the in situ pineal glands of golden hamsters and gerbils was examined using the avidin-biotin-peroxidase immunohistochemical technique. S-Ag was present in the gerbil pineal gland on the first postnatal day (P1), whereas it did not appear in the hamster pineal until P6. GFAP-immunoreactive astrocytes were first observed in the hamster pineal gland on P7 and in the gerbil pineal gland on P10. The number of S-Ag-immunoreactive pinealocytes and GFAP-immunoreactive astrocytes in the pineal glands of hamsters and gerbils increased with increasing age from P7 to 3 weeks. By 4 weeks, strong S-Ag and GFAP immunoreactivity was observed in both hamster and gerbil pineal glands. GFAP-immunoreactive stellate astrocytes were distributed evenly throughout the gerbil superficial pineal gland, but they were more often located in the peripheral region of the hamster superficial pineal. For the pineal grafts, pineal glands from neonatal (3-5 day old) hamsters were transplanted into the third cerebral ventricle (infundibular recess or posterior third ventricle) or beneath the renal capsule of adult male hamsters. S-Ag immunoreactivity appeared in the pineal grafts within 1 week following transplantation. By 4 weeks the pineal grafts showed strong S-Ag immunoreactivity which was maintained until at least 12 weeks after transplantation. The time course of glial cell maturation in the cerebroventricular pineal grafts is generally parallel to the hamster pineal gland in situ before 4 weeks. By 12 weeks, however, more astrocytes differentiated and developed GFAP-immunoreactivity in the pineal grafts than in the in situ pineals. These studies have described the postnatal development of S-Ag and GFAP immunoreactivity in in situ pineal glands and in neonatal pineal grafts.
采用抗生物素蛋白-生物素-过氧化物酶免疫组织化学技术，检测金黄仓鼠和沙鼠原位松果体中S-抗原和胶质纤维酸性蛋白（GFAP）免疫反应性的出生后发育情况。S-抗原在出生后第1天（P1）出现在沙鼠松果体中，而在仓鼠松果体中直到P6才出现。GFAP免疫反应性星形胶质细胞最早在仓鼠松果体中于P7被观察到，在沙鼠松果体中于P10被观察到。从P7到3周龄，仓鼠和沙鼠松果体中S-抗原免疫反应性松果体细胞和GFAP免疫反应性星形胶质细胞的数量随年龄增加而增加。到4周时，在仓鼠和沙鼠松果体中均观察到强烈的S-抗原和GFAP免疫反应性。GFAP免疫反应性星形胶质细胞均匀分布于沙鼠浅表松果体中，但它们更常位于仓鼠浅表松果体的周边区域。对于松果体移植，将新生（3 - 5日龄）仓鼠的松果体移植到成年雄性仓鼠的第三脑室（漏斗隐窝或第三脑室后部）或肾被膜下。移植后1周内，松果体移植物中出现S-抗原免疫反应性。到4周时，松果体移植物显示出强烈的S-抗原免疫反应性，并且至少维持到移植后12周。在4周之前，脑室松果体移植物中胶质细胞成熟的时间进程通常与仓鼠原位松果体平行。然而，到12周时，松果体移植物中分化并产生GFAP免疫反应性的星形胶质细胞比原位松果体中的更多。这些研究描述了原位松果体和新生松果体移植物中S-抗原和GFAP免疫反应性的出生后发育情况。
