Suárez I, Bodega G, Rubio M, Fernández B
Departamento de Biología Celular y Genética, Universidad de Alcalá, Madrid, Spain.
Glia. 1992;5(1):10-6. doi: 10.1002/glia.440050103.
Male and female hamsters aged 1, 4, and 10 postnatal weeks were used to study the distribution of vimentin and glial fibrillary acidic protein (GFAP) in the cerebellum. Vimentin immunoreactivity exceeded that of GFAP during the first postnatal week, although GFAP was also observed in all cerebellar layers. Immunoperoxidase analysis revealed that by the fourth postnatal week vimentin was only detected in Bergmann fibers and the very scarce fibrous astrocytes located in the inner white matter. The Purkinje cell bodies were only coated with GFAP-immunopositive processes. At 10 weeks, vimentin immunoreactivity was reduced to thin Bergmann glial processes, whereas GFAP immunoreactivity had greatly increased in the whole cerebellum. The GFAP immunostaining was denser in males than in females; however, in females, the Bergmann fibers were heavily immunostained with anti-vimentin in contrast to the males. The results described in the present paper indicate a sex difference in vimentin and GFAP immunoreactivities in the cerebellar astrocytes at 4 weeks of age, which persisted in the oldest hamsters in this study. The existence of sexual dimorphism might suggest that the expression of both gliofilament proteins could be influenced by circulating sex steroids.
选用出生后1周、4周和10周龄的雄性和雌性仓鼠，研究波形蛋白和胶质纤维酸性蛋白（GFAP）在小脑的分布。在出生后的第一周，波形蛋白的免疫反应性超过了GFAP，尽管在所有小脑层中也观察到了GFAP。免疫过氧化物酶分析显示，到出生后第四周，波形蛋白仅在伯格曼纤维和位于内白质中非常稀少的纤维性星形胶质细胞中检测到。浦肯野细胞体仅被GFAP免疫阳性的突起所覆盖。在10周时，波形蛋白的免疫反应性降低为纤细的伯格曼胶质突起，而GFAP的免疫反应性在整个小脑中大大增加。GFAP免疫染色在雄性中比在雌性中更密集；然而，与雄性相比，在雌性中，伯格曼纤维被抗波形蛋白强烈免疫染色。本文所述结果表明，4周龄时小脑星形胶质细胞中波形蛋白和GFAP免疫反应性存在性别差异，在本研究中最年长的仓鼠中这种差异仍然存在。性二态性的存在可能表明两种胶质丝蛋白的表达可能受循环性类固醇的影响。
