Baya A M, Toranzo A E, Lupiani B, Li T, Roberson B S, Hetrick F M
Department of Microbiology, University of Maryland, College Park 20742.
Appl Environ Microbiol. 1991 Nov;57(11):3114-20. doi: 10.1128/aem.57.11.3114-3120.1991.
A comparative analysis of the phenotypic and serological properties of Carnobacterium strains associated with mortalities of cultured striped bass and channel catfish and the properties of isolates from wild brown bullhead catfish in the Chesapeake Bay area in Maryland was conducted. All of the strains were gram-positive, facultatively anaerobic, nonmotile, non-spore-forming rods occurring singly or in short chains. They did not produce cytochrome oxidase or catalase, did not reduce nitrate, failed to produce H2S, were unable to grow on acetate medium, and did not produce gas from glucose or gluconate. The temperature and salinity ranges for most of the strains were 10 to 37 degrees C and 0 to 6% NaCl, respectively. The strains all fermented mannitol and inulin and were arginine dihydrolase positive; these are typical characteristics of Carnobacterium piscicola. The carbohydrate fermentation pattern exhibited by all of the isolates with the API-50 CHL system was also very similar to that shown by C. piscicola. Acid was produced from ribose, glucose, fructose, mannose, mannitol, N-acetylglucosamine, amygdaline, arbutin, esculin, salicin, cellobiose, maltose, sucrose, trehalose, and gentiobiose. The Carnobacterium strains did not show proteolytic, lipolytic, amylolytic, or hemolytic activity. Eighteen drugs were tested; all strains proved to be resistant to chloramphenicol, gentamicin, kanamycin, streptomycin, trimethoprim, quinolones, and nitrofurans. The analysis of membrane proteins supported the phenotypic similarities, two main patterns were established, one shared by the striped bass isolates and the reference strain of C. piscicola and another shared by most of the catfish strains. However, the agglutination assays demonstrated that only one Carnobacterium strain from striped bass was serologically related to C. piscicola ATCC 35586.(ABSTRACT TRUNCATED AT 250 WORDS)
对与养殖条纹鲈和沟鲶死亡事件相关的肉杆菌菌株的表型和血清学特性，以及马里兰州切萨皮克湾地区野生棕牛头鲶分离株的特性进行了比较分析。所有菌株均为革兰氏阳性、兼性厌氧、无运动性、不产芽孢的杆菌，单个或呈短链状存在。它们不产生细胞色素氧化酶或过氧化氢酶，不还原硝酸盐，不产生H2S，不能在醋酸盐培养基上生长，也不能从葡萄糖或葡萄糖酸盐中产气。大多数菌株的温度范围为10至37摄氏度，盐度范围为0至6% NaCl。这些菌株均发酵甘露醇和菊粉，精氨酸双水解酶呈阳性；这些是嗜鱼肉杆菌的典型特征。所有分离株在API-50 CHL系统中表现出的碳水化合物发酵模式也与嗜鱼肉杆菌非常相似。从核糖、葡萄糖、果糖、甘露糖、甘露醇、N-乙酰葡糖胺、扁桃苷、熊果苷、七叶苷、水杨苷、纤维二糖、麦芽糖、蔗糖、海藻糖和龙胆二糖中产酸。肉杆菌菌株未表现出蛋白水解、脂肪分解、淀粉分解或溶血活性。测试了18种药物；所有菌株均对氯霉素、庆大霉素、卡那霉素、链霉素、甲氧苄啶、喹诺酮类和硝基呋喃类耐药。膜蛋白分析支持表型相似性，建立了两种主要模式，一种由条纹鲈分离株和嗜鱼肉杆菌参考菌株共享，另一种由大多数鲶鱼菌株共享。然而，凝集试验表明，只有一株来自条纹鲈的肉杆菌菌株与嗜鱼肉杆菌ATCC 35586血清学相关。（摘要截断于250字）
