de Lederkremer R M, Zingales B, Confalonieri A N, Couto A S, Martin N F, Colli W
Biochem Int. 1985 Jan;10(1):79-88.
Incubation of epimastigote forms of Trypanosoma cruzi with (3H)-palmitic acid and (3H)-galactose, respectively, results in the incorporation of both precursors into the lipopeptidophosphoglycan (LPPG) and in at least two glycosphingolipids. Palmitic acid was incorporated into sphinganine and sphingenine, identified after hydrolysis, respectively, of the major and the minor glycosphingolipid. The purified glycosphingolipids labeled with either precursor migrated with a Rf similar to that of a sample labeled by periodate oxidation and borotritide reduction in which sialic acids have been previously characterized. This, together with the fact that the palmitic acid labeled glycosphingolipids were partially hydrolysed with neuraminidase favors a ganglioside-like structure for these compounds.
分别用（3H）-棕榈酸和（3H）-半乳糖培养克氏锥虫的上鞭毛体形式，结果这两种前体都被整合到脂肽磷糖（LPPG）以及至少两种糖鞘脂中。棕榈酸被整合到鞘氨醇和神经鞘氨醇中，这两种物质分别是在主要和次要糖鞘脂水解后鉴定出来的。用任一前体标记的纯化糖鞘脂迁移时的Rf值，与经高碘酸盐氧化和硼氚还原标记的样品相似，在该样品中唾液酸已被鉴定。这一点，再加上棕榈酸标记的糖鞘脂能被神经氨酸酶部分水解这一事实，表明这些化合物具有类似神经节苷脂的结构。
