Science. 1992 Mar 20;255(5051):1561-4. doi: 10.1126/science.255.5051.1561.
Dissolved organic matter (DOM) is the largest reservoir of reduced carbon in the oceans. The nature of DOM is poorly understood, in part, because it has been difficult to isolate sufficient amounts of representative material for analysis. Tangential-flow ultrafiltration was shown to recover milligram amounts of >1000 daltons of DOM from seawater collected at three depths in the North Pacific Ocean. These isolates represented 22 to 33 percent of the total DOM and included essentially all colloidal material. The elemental, carbohydrate, and carbon-type (by (13)C nuclear magnetic resonance) compositions of the isolates indicated that the relative abundance of polysaccharides was high ( approximately 50 percent) in surface water and decreased to approximately 25 percent in deeper samples. Polysaccharides thus appear to be more abundant and reactive components of seawater DOM than has been recognized.
溶解有机物（DOM）是海洋中还原碳的最大储存库。DOM 的性质还不太清楚，部分原因是因为很难分离出足够数量的有代表性的物质进行分析。切向流超滤法被证明可以从北太平洋三个深度采集的海水中回收毫克量的> 1000 道尔顿的 DOM。这些分离物占总 DOM 的 22%至 33%，并包含几乎所有胶体物质。分离物的元素、碳水化合物和碳型（通过（13）C 核磁共振）组成表明，多糖的相对丰度在表层水中较高（约 50%），在较深的样品中降低到约 25%。因此，多糖似乎是海水中 DOM 中比以前认为更丰富和更具反应性的成分。
