Liang Hao, Li Cuijuan, Yuan Qipeng, Vriesekoop Frank
State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, People's Republic of China.
J Agric Food Chem. 2008 Sep 10;56(17):7746-9. doi: 10.1021/jf801318v. Epub 2008 Aug 9.
In order to produce large amounts of pure sulforaphane for research purposes, a novel method using high-speed countercurrent chromatography (HSCCC) was developed. Without any initial cleanup steps, sulforaphane was successfully purified from the ethyl acetate extract of the broccoli seed meal by HSCCC. The separation was performed with two-phase solvent systems: n-hexane/ethyl acetate/methanol/water (1:5:1:5, v/v/v/v). From 850 mg of the ethyl acetate extract, 186 mg of sulforaphane was isolated with the solvent system. The purified compound was over 97% purity as determined by HPLC analysis, and the chemical structure was confirmed by MS and (1)H and (13)C NMR.
为了生产大量用于研究目的的纯萝卜硫素，开发了一种使用高速逆流色谱法（HSCCC）的新方法。无需任何初始净化步骤，通过HSCCC成功地从西兰花籽粕的乙酸乙酯提取物中纯化出萝卜硫素。分离使用两相溶剂系统进行：正己烷/乙酸乙酯/甲醇/水（1:5:1:5，v/v/v/v）。用该溶剂系统从850毫克乙酸乙酯提取物中分离出186毫克萝卜硫素。通过HPLC分析测定，纯化后的化合物纯度超过97%，其化学结构通过质谱、（1）H和（13）C核磁共振得以确认。
