Agar Nathalie Y R, Yang Hong Wei, Carroll Rona S, Black Peter M, Agar Jeffrey N
Department of Neurosurgery, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.
Anal Chem. 2007 Oct 1;79(19):7416-23. doi: 10.1021/ac071460e. Epub 2007 Sep 7.
Mass spectrometry imaging (MSI) acquires a grid of spatially resolved mass spectra and provides a molecular landscape of a tissue. This can have a myriad of uses: from basic tissue characterization to a comprehensive pathological diagnosis. We have developed a fast, inexpensive, histology-compatible tissue preparation method for matrix-assisted laser desorption/ionization (MALDI)-MSI, which overcomes current sample preparation-imposed limitations in image resolution. Tissue sections are prepared via simultaneous fixation and matrix deposition. This is accomplished by incorporating the MALDI matrix into solvents that preserve tissue integrity when applied according to standard histology procedures. This concept was expanded to include multiple histology protocols, thereby enabling analysis to be tailored to a variety of biomolecules and tissue types.
质谱成像（MSI）获取空间分辨质谱的网格，并提供组织的分子图谱。这有无数用途：从基础组织特征描述到全面的病理诊断。我们已经开发出一种用于基质辅助激光解吸/电离（MALDI）-MSI的快速、廉价且与组织学兼容的组织制备方法，该方法克服了当前样品制备对图像分辨率造成的限制。组织切片通过同时固定和基质沉积来制备。这是通过将MALDI基质掺入溶剂中来实现的，当按照标准组织学程序应用这些溶剂时，它们能保持组织完整性。这一概念已扩展到包括多种组织学方案，从而能够针对各种生物分子和组织类型进行定制分析。
