Yu Pengzhan, Li Xingqi, Li Xiunan, Lu Xiuling, Ma Guanghui, Su Zhiguo
National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, China.
Bioorg Med Chem Lett. 2007 Oct 15;17(20):5605-9. doi: 10.1016/j.bmcl.2007.07.094. Epub 2007 Aug 23.
A clear and powerful chromatographic approach to purify polyethylene glycol derivatives at a preparative scale was reported, which was based on the polystyrene-divinylbenzene beads with ethanol/water as eluants. The validity of this method was verified with the reaction mixture of mPEG-Glu and mPEG propionaldehyde diethylacetal (ALD-PEG) as the model. The target products were one-step achieved with the purity of >99% on the polymer resins column at gram scale. The method developed was free from such disadvantages as utility of toxic solvent and narrow application scope, which was combined with conventional approaches. The method developed provided an appealing and attractive alternative methods for purification of PEG derivatives at a preparative scale.
报道了一种清晰且高效的制备规模纯化聚乙二醇衍生物的色谱方法，该方法基于以乙醇/水为洗脱剂的聚苯乙烯-二乙烯基苯珠。以甲氧基聚乙二醇-谷氨酸（mPEG-Glu）和甲氧基聚乙二醇丙醛二乙缩醛（ALD-PEG）的反应混合物为模型验证了该方法的有效性。在聚合物树脂柱上以克规模一步获得纯度>99%的目标产物。所开发的方法没有使用有毒溶剂和应用范围窄等缺点，并且与传统方法相结合。所开发的方法为制备规模纯化聚乙二醇衍生物提供了一种有吸引力的替代方法。
