Alvestad Silje, Goa Pål Erik, Qu Hong, Risa Øystein, Brekken Christian, Sonnewald Ursula, Haraldseth Olav, Hammer Janniche, Ottersen Ole Petter, Håberg Asta
Department of Neuroscience, Norwegian University of Science and Technology (NTNU), N-7489 Trondheim, Norway.
Neuroimage. 2007 Oct 15;38(1):57-66. doi: 10.1016/j.neuroimage.2007.07.027. Epub 2007 Aug 7.
Mesial temporal lobe epilepsy is associated with structural and functional abnormalities, such as hippocampal sclerosis and axonal reorganization. The temporal evolution of these changes remains to be determined, and there is a need for in vivo imaging techniques that can uncover the epileptogenic processes at an early stage. Manganese-enhanced magnetic resonance imaging may be useful in this regard. The aim of this study was to analyze the temporospatial changes in manganese enhancement in rat brain during the development of epilepsy subsequent to systemic kainate application (10 mg/kg i.p.). MnCl(2) was given systemically on day 2 (early), day 15 (latent), and 11 weeks (chronic phase) after the initial status epilepticus. Twenty-four hours after MnCl(2) injection T1-weighted 3D MRI was performed followed by analysis of manganese enhancement. In the medial temporal lobes, there was a pronounced decrease in manganese enhancement in CA1, CA3, dentate gyrus, entorhinal cortex and lateral amygdala in the early phase. In the latent and chronic phases, recovery of the manganese enhancement was observed in all these structures except CA1. A significant increase in manganese enhancement was detected in the entorhinal cortex and the amygdala in the chronic phase. In the latter phase, the structurally intact cerebellum showed significantly decreased manganese enhancement. The highly differentiated changes in manganese enhancement are likely to represent the net outcome of a number of pathological and pathophysiological events, including cell loss and changes in neuronal activity. Our findings are not consistent with the idea that manganese enhancement primarily reflects changes in glial cells.
内侧颞叶癫痫与结构和功能异常有关，如海马硬化和轴突重组。这些变化的时间演变仍有待确定，因此需要能够在早期发现致痫过程的活体成像技术。锰增强磁共振成像在这方面可能有用。本研究的目的是分析在全身性应用红藻氨酸（10 mg/kg腹腔注射）后癫痫发展过程中大鼠脑内锰增强的时空变化。在首次癫痫持续状态后的第2天（早期）、第15天（潜伏期）和11周（慢性期）全身给予氯化锰。在注射氯化锰24小时后进行T1加权3D磁共振成像，随后分析锰增强情况。在内侧颞叶，早期CA1、CA3、齿状回、内嗅皮质和外侧杏仁核的锰增强明显降低。在潜伏期和慢性期，除CA1外，所有这些结构的锰增强均有恢复。在慢性期，内嗅皮质和杏仁核的锰增强显著增加。在后期，结构完整的小脑显示锰增强显著降低。锰增强的高度分化变化可能代表了许多病理和病理生理事件的最终结果，包括细胞丢失和神经元活动的变化。我们的发现与锰增强主要反映胶质细胞变化的观点不一致。
