Grenier Guillaume, Scimè Anthony, Le Grand Fabien, Asakura Atsushi, Perez-Iratxeta Carolina, Andrade-Navarro Miguel A, Labosky Patricia A, Rudnicki Michael A
Sprott Centre for Stem Cell Research, Ottawa Health Research Institute, 501 Smyth Road, Ottawa, Ontario K1H 8L6, Canada.
Stem Cells. 2007 Dec;25(12):3101-10. doi: 10.1634/stemcells.2006-0795. Epub 2007 Sep 6.
A novel population of tissue-resident endothelial precursors (TEPs) was isolated from small blood vessels in dermal, adipose, and skeletal muscle of mouse based on their ability to be grown as spheres. Cellular and molecular analyses of these cells revealed that they were highly related regardless of the tissue of origin and distinct from embryonic neural stem cells. Notably, TEPs did not express hematopoietic markers, but they expressed numerous characteristics of angiogenic precursors and their differentiated progeny, such as CD34, Flk-1, Tie-1, CD31, and vascular endothelial cadherin (VE-cadherin). TEPs readily differentiated into endothelial cells in newly formed vascular networks following transplantation into regenerating skeletal muscle. Taken together, these experiments suggest that TEPs represent a novel class of endothelial precursors that are closely associated with small blood vessels in muscle, adipose, and dermal tissue. This finding is of particular interest since it could bring new insight in cancer angiogenesis and collateral blood vessels developed following ischemia. Disclosure of potential conflicts of interest is found at the end of this article.
基于其形成球体生长的能力，从小鼠的真皮、脂肪和骨骼肌的小血管中分离出了一种新型的组织驻留内皮前体细胞（TEP）。对这些细胞的细胞和分子分析表明，无论其起源组织如何，它们都高度相关，并且与胚胎神经干细胞不同。值得注意的是，TEP不表达造血标志物，但它们表达了血管生成前体细胞及其分化后代的许多特征，如CD34、Flk-1、Tie-1、CD31和血管内皮钙黏蛋白（VE-钙黏蛋白）。将TEP移植到再生的骨骼肌中后，它们很容易在新形成的血管网络中分化为内皮细胞。综上所述，这些实验表明TEP代表了一类新型的内皮前体细胞，它们与肌肉、脂肪和真皮组织中的小血管密切相关。这一发现特别令人感兴趣，因为它可能为癌症血管生成以及缺血后形成的侧支血管带来新的见解。潜在利益冲突的披露见本文末尾。
