禾谷镰刀菌基因组揭示了局部多态性与病原体特化之间的联系。

The Fusarium graminearum genome reveals a link between localized polymorphism and pathogen specialization.

作者信息

Cuomo Christina A, Güldener Ulrich, Xu Jin-Rong, Trail Frances, Turgeon B Gillian, Di Pietro Antonio, Walton Jonathan D, Ma Li-Jun, Baker Scott E, Rep Martijn, Adam Gerhard, Antoniw John, Baldwin Thomas, Calvo Sarah, Chang Yueh-Long, Decaprio David, Gale Liane R, Gnerre Sante, Goswami Rubella S, Hammond-Kosack Kim, Harris Linda J, Hilburn Karen, Kennell John C, Kroken Scott, Magnuson Jon K, Mannhaupt Gertrud, Mauceli Evan, Mewes Hans-Werner, Mitterbauer Rudolf, Muehlbauer Gary, Münsterkötter Martin, Nelson David, O'donnell Kerry, Ouellet Thérèse, Qi Weihong, Quesneville Hadi, Roncero M Isabel G, Seong Kye-Yong, Tetko Igor V, Urban Martin, Waalwijk Cees, Ward Todd J, Yao Jiqiang, Birren Bruce W, Kistler H Corby

机构信息

Broad Institute of the Massachusetts Institute of Technology and Harvard, Cambridge, MA 02142, USA.

出版信息

Science. 2007 Sep 7;317(5843):1400-2. doi: 10.1126/science.1143708.

DOI:10.1126/science.1143708

PMID:17823352

Abstract

We sequenced and annotated the genome of the filamentous fungus Fusarium graminearum, a major pathogen of cultivated cereals. Very few repetitive sequences were detected, and the process of repeat-induced point mutation, in which duplicated sequences are subject to extensive mutation, may partially account for the reduced repeat content and apparent low number of paralogous (ancestrally duplicated) genes. A second strain of F. graminearum contained more than 10,000 single-nucleotide polymorphisms, which were frequently located near telomeres and within other discrete chromosomal segments. Many highly polymorphic regions contained sets of genes implicated in plant-fungus interactions and were unusually divergent, with higher rates of recombination. These regions of genome innovation may result from selection due to interactions of F. graminearum with its plant hosts.

摘要

我们对丝状真菌禾谷镰刀菌（栽培谷物的主要病原体）的基因组进行了测序和注释。检测到的重复序列极少，重复序列诱导点突变过程（其中重复序列会经历广泛突变）可能部分解释了重复序列含量的降低以及旁系同源（祖先重复）基因数量明显较少的现象。禾谷镰刀菌的另一菌株含有超过10000个单核苷酸多态性，这些多态性常常位于端粒附近以及其他离散的染色体片段内。许多高度多态性区域包含与植物 - 真菌相互作用相关的基因集，并且异常分化，重组率更高。这些基因组创新区域可能是由于禾谷镰刀菌与其植物宿主相互作用导致的选择结果。