Ugartondo Vanessa, Mitjans Montserrat, Touriño Sonia, Torres Josep Lluis, Vinardell María Pilar
Department of Physiology, Faculty of Pharmacy, Universitat de Barcelona, Spain.
Chem Res Toxicol. 2007 Oct;20(10):1543-8. doi: 10.1021/tx700253y. Epub 2007 Sep 8.
There is a great interest in characterizing the biological properties of natural compounds obtained from plants, especially polyphenols. We studied the structure-activity-cytotoxicity relationships of polyphenolic fractions obtained from grape pomace and pine bark. These fractions contained similar polymerised flavonoids but different percentages of pyrogallol groups that confer on them different biological properties. The human keratinocyte cell line HaCaT and the mouse fibroblast cell line 3T3 were used to study the cytotoxicity of the different fractions after 24, 48, and 72 h of exposure. Antioxidant activity of the fractions was evaluated by measuring the inhibition of hemolysis mediated by AAPH. Our results demonstrate that the polyphenolic fractions studied show high antioxidant capacity in a concentration range that is not harmful to normal human cells. Pine fractions presented slightly lower antioxidant activity than grape fractions but are less cytotoxic. This data provides useful information to help design safe antioxidant products that act without altering critical cell functions.
人们对表征从植物中获得的天然化合物，尤其是多酚的生物学特性有着浓厚的兴趣。我们研究了从葡萄皮渣和松树皮中获得的多酚组分的结构 - 活性 - 细胞毒性关系。这些组分含有相似的聚合黄酮类化合物，但邻苯三酚基团的百分比不同，这赋予了它们不同的生物学特性。使用人角质形成细胞系HaCaT和小鼠成纤维细胞系3T3来研究不同组分在暴露24、48和72小时后的细胞毒性。通过测量AAPH介导的溶血抑制来评估组分的抗氧化活性。我们的结果表明，所研究的多酚组分在对正常人类细胞无害的浓度范围内显示出高抗氧化能力。松树组分的抗氧化活性略低于葡萄组分，但细胞毒性较小。这些数据提供了有用的信息，有助于设计在不改变关键细胞功能的情况下起作用的安全抗氧化产品。
